Complement Factor H Related Protein 1 (CFHR1) ELISA Kit | MyBioSource MBS2000009 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Complement Factor H Related Protein 1 (CFHR1) ELISA Kit

catalog :

MBS2000009

quantity :

48-Strip-Wells

price :

610 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2000009

products type :

ELISA Kit

products full name :

Complement Factor H Related Protein 1 (CFHR1) ELISA Kit

products short name :

Complement Factor H Related Protein 1 (CFHR1)

products name syn :

CFHL; CFHL1; CFHL1P; CFHR1P; FHR1; H36-1; H36-2; HFL1; HFL2; H factor-like protein 1

other names :

complement factor H-related protein 1; Complement factor H-related protein 1; complement factor H-related protein 1; complement factor H-related 1; H factor-like protein 1; H-factor-like 1; H36

products gene name :

CFHR1

other gene names :

CFHR1; CFHR1; CFHL; FHR1; HFL1; HFL2; CFHL1; H36-1; H36-2; CFHL1P; CFHR1P; CFHL; CFHL1; CFHL1P; CFHR1P; FHR1; HFL1; HFL2; FHR-1; H-factor-like 1

uniprot entry name :

FHR1_HUMAN

reactivity :

Human

sequence length :

330

specificity :

This assay has high sensitivity and excellent specificity for detection of Complement Factor H Related Protein 1 (CFHR1). No significant cross-reactivity or interference between Complement Factor H Related Protein 1 (CFHR1) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Typically less than 0.293ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor H Related Protein 1 (CFHR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor H Related Protein 1 (CFHR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H Related Protein 1 (CFHR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor H Related Protein 1 (CFHR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor H Related Protein 1 (CFHR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Complement Factor H Related Protein 1 (CFHR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

118442839

ncbi acc num :

NP_002104.2

ncbi gb acc num :

NM_002113.2

ncbi mol weight :

37,651 Da

ncbi summary :

This gene encodes a secreted protein belonging to the complement factor H protein family. It binds to Pseudomonas aeruginosa elongation factor Tuf together with plasminogen, which is proteolytically activated. It is proposed that Tuf acts as a virulence factor by acquiring host proteins to the pathogen surface, controlling complement, and facilitating tissue invasion. Mutations in this gene are associated with an increased risk of atypical hemolytic-uremic syndrome. [provided by RefSeq, Oct 2009]

uniprot summary :

CFHR1: Might be involved in complement regulation. Can associate with lipoproteins and may play a role in lipid metabolism. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular space. Biological Process: complement activation. Disease: Macular Degeneration, Age-related, 1; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 1

size1 :

48-Strip-Wells

price1 :

610 USD

size2 :

96-Strip-Wells

price2 :

830

size3 :

5x96-Strip-Wells

price3 :

3265

size4 :

10x96-Strip-Wells

price4 :

5870

more info or order :

MyBioSource product webpage