protein

Recombinant Human Tumor Necrosis Factor-alpha (rHuTNF- alpha)

MBS197020

0.01 mg

205 USD

Recombinant Protein

Recombinant Human Tumor Necrosis Factor-alpha (rHuTNF- alpha)

Tumor Necrosis Factor - alpha (rHuTNF-a)

Recombinant Human Tumor Necrosis Factor - alpha (rHuTNF-a)

tumor necrosis factor; Tumor necrosis factor; tumor necrosis factor; TNF-a; cachectin; APC1 protein; OTTHUMP00000029281; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2

TNF; TNF; DIF; TNFA; TNFSF2; TNF-alpha; TNFA; TNFSF2

TNFA_HUMAN

E Coli

233

Recombinant Human Tumor Necrosis Factor - alpha (rHuTNF-a)

Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain. Purification: Chromatographic

Sterile filtered, lyophilized.

Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20 degree C. Upon reconstitution, store at 4 degree C for 2-7 days or aliquot and store at -20 degree C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.

Dimers/Aggregates: <1% by SDSPAGE. Biological Activity: Fully biologically active compared to reference standards. Specific Activity 2 x 10^7 IU/mg.

Endotoxin Level: <0.1 ng/ug . Protein Content: Determined spectrophotometrically at 280nm (extinction coefficient 1.234) and by RPHPLC analysis against a calibrated standard. Solubility: Reconstitute the lyophilized product in sterile water not less than 100 ug/ml. Dialyzed against 5 mM Tris pH 8 prior to lyophilization. Stabilizer: None. Preservatives: None

Recombinant Proteins

Recombinant Human TNF- alpha produced in E. coli is a single, nonglycosylated, polypeptide chain containing 158 amino acids with a molecular mass of 17,484 daltons. This product has full biological activity.

25952111

NP_000585.2

NM_000594.2

P01375

25,644 Da

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This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Subunit structure: Homotrimer. Subcellular location: Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor, soluble form: Secreted. Post-translational modification: The soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. Ref.18 Ref.19O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. Ref.15. Polymorphism: Genetic variations in TNF influence susceptibility to hepatitis B virus (HBV) infection [. MIM:610424]. Involvement in disease: Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [. MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Sequence similarities: Belongs to the tumor necrosis factor family. Sequence caution: The sequence AAF71992.1 differs from that shown. Reason: Frameshift at positions 91 and 157. The sequence CAA75070.1 differs from that shown. Reason: Erroneous gene model prediction.

0.01 mg

205 USD