protein

Recombinant Human Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha)

MBS197017

0.005 mg

195 USD

Recombinant Protein

Recombinant Human Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha)

Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha)

Recombinant Human Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha)

macrophage inflammatory protein 1 alpha; C-C motif chemokine 3; C-C motif chemokine 3; SIS-beta; PAT 464.1; OTTHUMP00000163953; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a); chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha proteinCleaved into the following chain:MIP-1-alpha(4-69); Alternative name(s):; LD78-alpha(4-69)

CCL3; CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha; G0S19-1; MIP1A; SCYA3

CCL3_HUMAN

E Coli

92

Recombinant Human Macrophage Inflammatory Protein-1 alpha (rHuMIP-1 alpha)

Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain. Purification: Chromatographic

Sterile filtered, lyophilized.

Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20 degree C. Upon reconstitution, store at 4 degree C for 2-7 days or aliquot and store at -20 degree C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.

Dimers/Aggregates: <1% by SDSPAGE. Biological Activity: Fully biologically active compared to reference standards. Activity determined by the ability to chemoattract human monocytes at a concentration of 1-10 ng/ml.

Endotoxin Level: <0.1 ng/ug . Protein Content: Determined spectrophotometrically at 280nm (extinction coefficient 0.9) and by RPHPLC analysis against a calibrated standard. Solubility: Reconstitute the lyophilized product in sterile water not less than 100 ug/ml . Stabilizer: None. Preservatives: None

Recombinant Proteins

Recombinant Human MIP-1 alpha produced in E. coli is a single, nonglycosylated, polypeptide chain containing 70 amino acids with a molecular mass of 7,820 daltons. This product has full biological activity.

2905628

AAC03539.1

P10147

10,085 Da

Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); GPCR Ligand Binding Pathway (161020); IL12-mediated Signaling Events Pathway (137922)

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted, signal peptide; Chemokine; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol. Molecular Function: identical protein binding; CCR1 chemokine receptor binding; protein binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; chemoattractant activity; CCR5 chemokine receptor binding; phospholipase activator activity; protein kinase activity. Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; behavior; response to toxin; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; regulation of cell shape; negative regulation of bone mineralization; monocyte chemotaxis; cell-cell signaling; positive chemotaxis; calcium ion transport; positive regulation of neuron apoptosis; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; calcium-mediated signaling; MAPKKK cascade; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; macrophage chemotaxis; cellular calcium ion homeostasis; osteoblast differentiation; cell activation; positive regulation of protein kinase B signaling cascade; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of cell migration; positive regulation of inflammatory response. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

0.005 mg

195 USD

0.02 mg

280

1 mg

3295