catalog number :
MBS197016
products type :
Recombinant Protein
products full name :
Recombinant Human Leptin
products short name :
Leptin (Anti-Obesity Protein) (rHuLeptin)
products name syn :
Recombinant Human Leptin (Anti-Obesity Protein) (rHuLeptin)
other names :
leptin; leptin; obesity homolog; leptin
other gene names :
lep; ob; xlep
specificity :
Recombinant Human Leptin (Anti-Obesity Protein) (rHuLeptin)
purity :
Greater than 95% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain. Purification: Chromatographic
form :
Sterile filtered, lyophilized.
storage stability :
Lyophilized product is stable at room temperature, but should be stored desiccated at -20 degree C. Upon reconstitution, store at 4 degree C. Avoid repeated freeze-thaw cycles.
other info1 :
Dimers/Aggregates: <1% by SDSPAGE. Biological Activity: Fully biologically active compared to reference standards. ED50 determined by the leptin dependent stimulation of human OB-R transfected murine BaF3 cells is 0.5-1.6 ng/ml.
other info2 :
Endotoxin Level: <0.1 ng/ug. Protein Content: Determined spectrophotometrically at 280nm (extinction coefficient 0.878) and by RPHPLC analysis against a calibrated standard. Solubility: Soluble in water and most aqueous buffers above and below the isoelectric point. Dialyzed against 45mM sodium bicarbonate prior to lyophilization. Stabilizer: None. Preservatives: None
products categories :
Recombinant Proteins
products description :
Recombinant Human Leptin produced in E. coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids with a molecular mass of 16,240 daltons. This product has full biological activity.
ncbi acc num :
NP_001089183.1
ncbi gb acc num :
NM_001095714.1
ncbi pathways :
Adipocytokine Signaling Pathway (119298); Adipocytokine Signaling Pathway (505); Cytokine-cytokine Receptor Interaction Pathway (119282); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (84661); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (119283); Neuroactive Ligand-receptor Interaction Pathway (462)