protein

Recombinant Human Insulin

MBS197009

25 mg

270 USD

Recombinant Protein

Recombinant Human Insulin

Insulin (rHuInsulin)

Recombinant Human Insulin (rHuInsulin)

insulin; Insulin; insulin; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162; OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519; insulin

INS; INS; ILPR; IRDN; IDDM2; MODY10

INS_HUMAN

E Coli

Recombinant Human Insulin (rHuInsulin)

Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain. Purification: Chromatographic

Lyophilized Insulin is stable at room temperature for 3 weeks, but it is recommended that it is store desiccated at -20 degree C. After reconstitution, it can be stored at 4 degree C for 2-7 days but should be stored below -20 degree C after that. For long-term storage of reconstituted Insulin, it is recommended that a carrier protein (such as 0.1% HAS or BSA) is added.

Dimers/Aggregates: <1% by SDSPAGE

Endotoxin Level: <0.1 ng/ug. Protein Content: Determined spectrophotometrically at 280nm, and by RP-HPLC analysis against a calibrated insulin standard.Stabilizer: None. Preservatives: None. Physical Appearance: White lyophilized powder. Formulation: Sterile-filtered, lyophilized from a 1mg/ml solution with no additives. Solutbility: Reconstitute in sterile 0.005N HCl to a concentration of no more than 1 mg/ml. Biological Activity: 28U/mg

Recombinant Proteins

Recombinant Human Insulin produced in E. coli is a two chain, nonglycosylated polypeptide chain containing 51 amino acids with a molecular mass of 5,807 daltons. This product has full biological activity. Provided as a lyophilized powder.

386828

P01308

11,981 Da

ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Amyloids Pathway (366238); Arf6 Trafficking Events Pathway (137954); Diabetes Pathways (105902); Downstream Signaling Of Activated FGFR Pathway (160957); FOXA1 Transcription Factor Network Pathway (137979); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family. Protein type: Hormone; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region. Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding. Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; negative regulation of protein oligomerization; positive regulation of glucose import; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; positive regulation of nitric-oxide synthase activity; activation of protein kinase B; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of amino acid metabolic process; negative regulation of protein catabolic process; positive regulation of cell differentiation; negative regulation of glycogen catabolic process; wound healing; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; glucose transport; regulation of protein secretion; negative regulation of lipid catabolic process; positive regulation of MAPKKK cascade; negative regulation of vasodilation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; alpha-beta T cell activation; negative regulation of fatty acid metabolic process; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; energy reserve metabolic process; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration. Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10

25 mg

270 USD

250 mg

530

1 g

1385