antibody

Protein Disulfide Isomerase (PDI) Polyclonal Antibody

MBS194487

0.1 ml

420 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Antibody

Protein Disulfide Isomerase (PDI) Polyclonal Antibody

PDI

protein disulfide isomerase; Protein disulfide-isomerase; protein disulfide-isomerase; p55; OTTHUMP00000196834; protocollagen hydroxylase; prolyl 4-hydroxylase subunit beta; collagen prolyl 4-hydroxylase beta; thyroid hormone-binding protein p55; glutathione-insulin transhydrogenase; cellular thyroid hormone-binding protein; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase family A, member 1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase, beta polypeptide; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55

PDI

P4HB; P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta; ERBA2L; PDI; PDIA1; PO4DB

PDIA1_HUMAN

Polyclonal

IgG

Rabbit

Human, monkey, mouse, rat, hamster, bovine, ovine, porcine, canine, guinea pig, xenopus

PDI. This antibody recognizes human, monkey, mouse, rat, hamster, bovine, ovine, porcine, canine, guinea pig, and Xenopus PDI.

100ul whole antiserum

Store frozen product at or below -20 degree C. For optimal storage, aliquot to smaller portions and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Immunohistochemistry; Immunoprecipitation; Immunoprecipitation; Western Blot; Immunocytochemistry

Western blot: Use at 1:4,000 dilution. A band of approx. 58 kD is detected. Immunocytochemistry/Immunohistochemistry: Use at 2-5 ug/ml. Immunoprecipitation: Use at 10 ug/ml.

Antigen: Synthetic peptide derived from rat PDI conjugated to KLH. Preservative: None. Available on request.

Dilution Instructions: Dilute in PBS or medium that is identical to that used in the assay system.

A microsomal enzyme known as Protein Disulfide Isomerase (PDI) is involved in the formation of disulfide bonds in many extracellular proteins through its oxidase and isomerase activities. It is also involved in the reduction of disulfide bonds in proteins. PDI is located primarily in the endoplasmic reticulum (ER) but may also be found in the cytosol. PDI can reside in the ER due to the highly conserved KDEL sequence at its carboxy terminus which serves as an ER-retention signal.

13540806

P07237

57,116 Da

Chylomicron-mediated Lipid Transport Pathway 106157!!Lipid Digestion, Mobilization, And Transport Pathway 106111!!Lipoprotein Metabolism Pathway 106156!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!Protein Processing In Endoplasmic Reticulum Pathway 167325!!Protein Processing In Endoplasmic Reticulum Pathway 167190

This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq]

Function: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Ref.17 Ref.19. Catalytic activity: Catalyzes the rearrangement of -S-S- bonds in proteins. Subunit structure: Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. By similarity. Binds UBQLN1. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex. Ref.20. Subcellular location: Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein. Potential. Note: Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. Probable. Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.17 Ref.18 Ref.23 Ref.27. Miscellaneous: Reduces and may activate fusogenic properties of HIV-1 gp120 surface protein, thereby enabling HIV-1 entry into the cell. Sequence similarities: Belongs to the protein disulfide isomerase family.Contains 2 thioredoxin domains.

0.1 ml

420 USD