catalog number :
MBS194477
products full name :
Biliverdin Reductase Polyclonal Antibody
products short name :
Billverdin Reductase
products name syn :
Billverdin Reductase; Biliverdin Reductase
specificity :
Billverdin Reductase [Rabbit polyclonal antibody to Biliverdin Reductase]. This antibody detects a 41-42 kDa protein in human samples and a 33 kDa protein in rat samples on SDS-PAGE immunoblots, corresponding to the apparent molecular weight of biliverdin reductase. Human and rat biliverdin reductase share some antigenic epitopes, and some immunochemical cross-reactivity is seen. This antibody reacts with human, mouse, rat, hamster and pig biliverdin reductase.
form :
Supplied as Protein A-purified antibody in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
storage stability :
Store at or below -20 degree C. Avoid repeated freeze/thaw cycles.
tested application :
Western Blot, Immunohistochemistry, Immunocytochemistry
app notes :
Western Blot: Use at 1-2 ug/ml
other info1 :
Antigen: Recombinant rat liver biliverdin reductase.
other info2 :
Dilution Instructions: Dilute in PBS or medium that is identical to that used in the assay system.
products categories :
Enzyme and Enzyme Inhibitor Antibodies
products description :
In mammalian species, biliverdin reductase (BVR) converts tetrapyrrole to bilirubin. BVR is a unique enzyme in that it has two pH optima (6.8 and 8.7) and uses different cofactors at each pH optimum: NADH at pH 6.8 and NADPH at pH 8.7. BVR displays pl and molecular mass microheterogeneity which are likely a result of post-translational modifications. BVR is not inactivated by heat shock, and its microheterogeniety is preserved under thermal stress. Human BVR is a longer polypeptide than the rat equivalent (41-42kDa vs. 33kDa), although human and rat BVR share some epitopes and show immunochemical cross-reactivity.