antibody

Ubiquitin Polyclonal Antibody

MBS194372

0.05 mg

285 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry, immunoprecipitation

Antibody

Ubiquitin Polyclonal Antibody

Ubiquitin

Ubiquitin Antibody; Ubiquitin

ubiquitin; Ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a; ubiquitin C; ubiquitin-CEP80; OTTHUMP00000200786; OTTHUMP00000200788; OTTHUMP00000200789; OTTHUMP00000201692; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80; ribosomal protein S27a; Ubiquitin carboxyl extension protein 80

RPS27A; RPS27A; UBC; S27A; CEP80; UBA80; UBCEP1; UBCEP80; UBA80; UBCEP1

RS27A_HUMAN

Polyclonal

Rabbit

Ubiquitin Antibody [Rabbit polyclonal antibody to Ubiquitin]. This antibody detects a 10 kDa protein on SDS-PAGE immunoblots, corresponding to the molecular weight of ubiquitin. This antibody will identify free ubiquitin as well as ubiquinated proteins on western blot. This antibody reacts with human, monkey, mouse, rat, hamster, rabbit guinea, pig, bovine, pig, canine, sheep, chicken, Xenopus, yeast, Drosophila, E. coli, and fish ubiquitin.

Antiserum

Store frozen product at or below -20 degree C. Thawed product may be stored for 2-4 weeks at 4 degree C. For optimal storage, aliquot and store at -20 degree C.

Western Blot, Immunohistochemistry, Immunoprecipitation

Western Blot: 1:1,000. Immunoprecipitation: 1:100. Immunohistochemistry: 1:200

Antigen: Ubiquitin purified from bovine red blood cells.

Apoptosis Antibodies

37571

P62979

17,965 Da

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 105825!!APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway 105832!!APC/C:Cdc20 Mediated Degradation Of Securin Pathway 105833!!APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105831!!APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway 105835!!Activated TLR4 Signalling Pathway 106400!!Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105829!!Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway 105970!!Adaptive Immunity Signaling Pathway 366160!!Antigen Processing: Ubiquitination Proteasome Degradation Pathway 366162

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq]

Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Ref.12 Ref.19Ribosomal protein S27a is a component of the 40S subunit of the ribosome. Ref.12 Ref.19. Subunit structure: Ribosomal protein S27a is part of the 40S ribosomal subunit. Subcellular location: Ubiquitin: Cytoplasm. By similarity. Nucleus. Miscellaneous: Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.For a better understanding, features related to ubiquitin are only indicated for the first chain. Sequence similarities: In the N-terminal section; belongs to the ubiquitin family.In the C-terminal section; belongs to the ribosomal protein S27Ae family.Contains 1 ubiquitin-like domain.

0.05 mg

285 USD