Retinoblastoma (Rb) phosphothreonine 826 Polyclonal Antibody | MyBioSource MBS194348 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Retinoblastoma (Rb) phosphothreonine 826 Polyclonal Antibody

catalog :

MBS194348

quantity :

0.025 mg

price :

395 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS194348

products type :

Antibody

products full name :

Retinoblastoma (Rb) phosphothreonine 826 Polyclonal Antibody

products short name :

Retinoblastoma phosphothreonine 826

products name syn :

Retinoblastoma phosphothreonine 826 polyclonal antibody

other names :

retinoblastoma; Retinoblastoma-associated protein; retinoblastoma-associated protein; OTTHUMP00000018397; retinoblastoma suspectibility protein; retinoblastoma 1; p105-Rb; pRb; Rb; pp110

other gene names :

RB1; RB1; RB; pRb; OSRC; pp110; p105-Rb

uniprot entry name :

RB_HUMAN

clonality :

Polyclonal

host :

Rabbit

specificity :

Retinoblastoma phosphothreonine 826 polyclonal antibody [Rabbit polyclonal antibody specific for the phosphorylated form of human retinoblastoma protein at threonine 826. Useful in WB. Available as affinity purified antibody.]. This antibody reacts with human Rb protein phosphorylated at serine 826. Mouse andrat (100% conserved) have not been tested.

form :

Sequential immunoaffinity-purified antibody in phosphate buffer, pH 7.4

storage stability :

Store antibody at -80 degree C. Upon initial thawing, aliquot in appropriate aliquots and store at -80 degree C. Avoid multiple freeze-thaw cycles.

tested application :

Western Blot

app notes :

Western Blotting: 0.25-1.0 ug/ml is recommended. The optimal antibody concentrationshould be determined for each specific application.

other info1 :

Antigen: Phosphopeptide region of human Rb containing serine 826

other info2 :

Positive Control: Jurkat cell lysate in high growth phase. Stabilizer: 1 mg/ml BSA. Preservatives: 0.05% Sodium Azide

products categories :

Cancer Research Products

ncbi gi num :

29123599

uniprot acc num :

P06400

ncbi mol weight :

106,159 Da

ncbi pathways :

Adipogenesis Pathway (198832); Androgen Receptor Signaling Pathway (198806); B Cell Receptor Signaling Pathway (198909); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chronic Myeloid Leukemia Pathway (83116)

ncbi summary :

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]

uniprot summary :

Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression. Protein type: Oncoprotein; Transcription, coactivator/corepressor; Tumor suppressor; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 13q14.2. Cellular Component: nucleoplasm; SWI/SNF complex; Rb-E2F complex; PML body; spindle; nucleus; chromatin. Molecular Function: identical protein binding; protein binding; androgen receptor binding; DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; transcription factor binding; kinase binding; transcription factor activity. Biological Process: viral reproduction; sister chromatid biorientation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter, mitotic; regulation of mitotic cell cycle; neuron maturation; cell cycle arrest; positive regulation of macrophage differentiation; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; mitotic cell cycle checkpoint; negative regulation of transcription factor activity; enucleate erythrocyte differentiation; regulation of lipid kinase activity; myoblast differentiation; chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; Ras protein signal transduction; androgen receptor signaling pathway; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; cell cycle checkpoint; G1/S transition of mitotic cell cycle. Disease: Bladder Cancer; Osteogenic Sarcoma; Small Cell Cancer Of The Lung; Retinoblastoma

size1 :

0.025 mg

price1 :

395 USD

more info or order :

MyBioSource product webpage