catalog number :
MBS194216
products full name :
Cytochrome P450 Reductase Polyclonal Antibody
products short name :
Cytochrome P450 Reductase
products name syn :
Cytochrome P450 Reductase; Cytochrome P450 Reductase
other names :
NADPH--cytochrome P450 reductase; NADPH--cytochrome P450 reductase; NADPH--cytochrome P450 reductase; OTTHUMP00000160964; OTTHUMP00000185560; OTTHUMP00000210071; NADPH-dependent cytochrome P450 reductase; P450 (cytochrome) oxidoreductase
other gene names :
POR; POR; CPR; CYPOR; P450R; FLJ26468; DKFZp686G04235; CYPOR
uniprot entry name :
NCPR_HUMAN
specificity :
Cytochrome P450 Reductase [Rabbit polyclonal antibody to Cytochrome P450 Reductase]. This antibody detects a 78 kDa protein on SDS-PAGE immunoblots, corresponding to the molecular weight of cytochrome P450 reductase. This antibody reacts with human, monkey, bovine, mouse, rat, canine, guinea pig, hamster, pig, sheep and rabbit cytochrome P450 reductase
storage stability :
Store frozen product at or below -20 degree C. Thawed product may be stored for 2-4 weeks at 4 degree C. For optimal storage, aliquot and store at -20 degree C.
tested application :
Western Blot, Immunocytochemistry, Immunoprecipitation
app notes :
Western Blot: 1:1,000. Immunocytochemistry: 1:500 - 1:1,000. Immunoprecipitation: 1:100
other info1 :
Antigen: Purified rat liver NADPHCytochrome P450 reductase.
products categories :
Enzyme and Enzyme Inhibitor Antibodies
ncbi acc num :
NP_000932.3
ncbi gb acc num :
NM_000941.2
ncbi mol weight :
76,690 Da
ncbi pathways :
1,25-dihydroxyvitamin D3 Biosynthesis Pathway 138457
ncbi summary :
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]
uniprot summary :
Function: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Catalytic activity: NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein. Cofactor: FAD.FMN. Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region. Involvement in disease: Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [. MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome. Ref.10 Ref.11 Ref.12 Ref.13Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [. MIM:201750]. Ref.13. Sequence similarities: In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain. Sequence caution: The sequence AAH34277.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.