antibody

Anti-Survivin (NT) Antibody

MBS194145

0.1 mg

370 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-Survivin (NT) Antibody

Survivin (NT)

Anti-Survivin (NT); Survivin (NT)

baculoviral IAP repeat-containing protein 5; Baculoviral IAP repeat-containing protein 5; baculoviral IAP repeat-containing protein 5; survivin; apoptosis inhibitor survivin; Apoptosis inhibitor survivin

BIRC5; BIRC5; BIRC5

BIRC5_CANFA

Rabbit

Anti-Survivin (NT) [Rabbit antibody to peptide corresponding to aa 1-12 of human survivin.]. This antibody recognizes human survivin (17 kD).

100 ug IgG in 200 ul PBS, pH 7.4, purified by immunoaffinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Immunoblot

Immunoblotting: use at 1:500-1:1,000 dilution. Positive control: MOLT4 cells.

Antigen: Peptide corresponding to aa 1-12 of human survivin.

Stabilizer: None. Preservatives: 0.02% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Apoptosis Antibodies

Apoptosis is related to many diseases and is triggered by a variety of stimuli including members of the TNF family. Apoptosis can be prevented by the inhibitor of apoptosis proteins (IAPs). IAPs constitute a conserved gene family that binds to and inhibits cell death proteases. A novel IAP has been identified and designated survivin. Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity. Survivin is expressed predominantly in tissues of embryos, transformed cell lines, and many human cancers.

50978646

NP_001003019.1

NM_001003019.1

Q8I009

16,271 Da

Colorectal Cancer Pathway (84152); Colorectal Cancer Pathway (518); Pathways In Cancer (84151)

Function: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Subunit structure: Monomer or homodimer. Exist as a homodimer in the apo state and as a monomer in the CPC-bound state. By similarity. When phosphorylated, interacts with HBXIP; the resulting complex binds pro-caspase-9, as well as active caspase-9, but much less efficiently. Component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with JTB. Interacts with CDCA8 and INCENP; interaction is direct. Interacts with EVI5. Interacts with GTP-bound RAN in both the S and M phases of the cell cycle. Interacts with USP9X. Interacts with tubulin. Subcellular location: Cytoplasm. By similarity. Nucleus. By similarity. Chromosome centromere. By similarity. Cytoplasm cytoskeleton spindle. By similarity. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. By similarity. UniProtKB O15392. Domain: The BIR repeat is necessary and sufficient for HBXIP binding. Post-translational modification: Ubiquitination is required for centrosomal targeting. By similarity.In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes. Sequence similarities: Belongs to the IAP family.Contains 1 BIR repeat.

0.1 mg

370 USD