antibody

Anti-SIRP alpha (CT) Antibody

MBS194138

0.1 mg

360 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-SIRP alpha (CT) Antibody

SIRP alpha (CT)

Anti-SIRP alpha (CT); SIRPa (CT)

tyrosine-protein phosphatase non-receptor type substrate 1; Tyrosine-protein phosphatase non-receptor type substrate 1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; OTTHUMP00000030001; OTTHUMP00000159968; OTTHUMP00000159969; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; signal-regulatory protein alpha; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84

SIRPA; SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP

SHPS1_HUMAN

Polyclonal

Rabbit

Anti-SIRP alpha (CT) [Polyclonal (rabbit) antibody to peptide sequence at C-terminus of human SIRPa, a substrate for many activated tyrosine kinases.]. This antibody recognizes full-length human SIRP alpha (75-110 kD). The sequence of this peptide differs from those of mouse, rat, and bovine SIRP alpha by one amino acid, so this antibody may be used to detect SIRP alpha of these species.

100 ug IgG in PBS, pH 7.4, purifiedby immunoaffinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Immunoblot

Immunoblotting : use a1:1,000-1:2,000 dilution. Positive control: Whole cell lysate from THP-1 cells.

Antigen: Peptide corresponding to the Cterminus of human SIRP alpha .

Stabilizer: None. Preservatives: 0.02% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Neuroscience and Signal Transduction Antibodies

Protein tyrosine phosphatases (PTPases), such as SHP-1 and SHP-2, are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse, and cattle by several laboratories and was designated SIRP alpha, SHPS-1, and MyD-1. SIRP alpha/SHPS-1 is a new gene family containing at least fifteen members. SIRP alpha/SHPS-1 is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR, and src, and is a specific docking protein for SHP-2. SIRP alpha/SHPS-1 has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, and cell adhesion and plays a general role in different physiological and pathological processes.

18426911

NP_542970.1

NM_080792.2

P78324

54,967 Da

Cell Junction Organization Pathway 160966!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Hemostasis Pathway 106028!!IL-1 Signaling Pathway 198908!!Interactions Of The Immunoglobulin Superfamily (IgSF) Member Proteins Pathway 187227!!Osteoclast Differentiation Pathway 193147!!Osteoclast Differentiation Pathway 193096!!Signal Regulatory Protein (SIRP) Family Interactions Pathway 187209

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq]

Function: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. By similarity. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Ref.8 Ref.10. Subunit structure: Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. By similarity. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Subcellular location: Membrane; Single-pass type I membrane protein. Tissue specificity: Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. Post-translational modification: N-glycosylated. Ref.2 Ref.11 Ref.13Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. Ref.2 Ref.9 Ref.12 Ref.14. Sequence similarities: Contains 2 Ig-like C1-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

0.1 mg

360 USD