Anti-MADD Antibody | MyBioSource MBS194115 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-MADD Antibody

catalog :

MBS194115

quantity :

0.1 mg

price :

370 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS194115

products type :

Antibody

products full name :

Anti-MADD Antibody

products short name :

MADD (CT)

products name syn :

Anti-MADD (CT); MADD

other names :

MAP kinase-activating death domain protein isoform j; DENN protein; MAP kinase-activating death domain protein; OTTHUMP00000197131; OTTHUMP00000197132; OTTHUMP00000197133; OTTHUMP00000197134; OTTHUMP00000197135; OTTHUMP00000197136; OTTHUMP00000197137; OTTHUMP00000237191; OTTHUMP00000238029; Rab3 GDP/GTP exchange factor; insulinoma glucagonoma clone 20; differentially expressed in normal and neoplastic cells; MAP-kinase activating death domain

other gene names :

MADD; DENN; DENN; IG20; RAB3GEP; FLJ35600; FLJ36300; KIAA0358

uniprot entry name :

Q7KYV8_HUMAN

host :

Rabbit

specificity :

Anti-MADD (CT) [Rabbit antibody to peptide corresponding to aa 1570-1588 of human MADD. Recognizes human and mouse MADD.]. This antibody recognizes human and mouse MADD (200-220 kD).

form :

100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma-tography.

storage stability :

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

tested application :

Immunoblot

app notes :

Immunoblotting : use at 1:250-1:500 dilution. Positive control: Whole cell lysate from HeLa or NIH3T3 cells.

other info1 :

Antigen: Peptide corresponding to aa 1570-1588 of human MADD. This peptide sequence is identical to that of DENN and differs by one amino acid with rat GDP/GTP exchange protein RAB3-GEP.

other info2 :

Stabilizer: None. Preservatives: 0.02% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

products categories :

Apoptosis Antibodies

products description :

MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1). Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% homology with DENN (differentially expressed in neoplasic vs. normal cells) which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greather than 94% homology with a GDO/GTP exchange protein , Rab3-GEP, and it is 87% homologous with KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signalling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.

ncbi gi num :

209863002

ncbi acc num :

NP_001129416.1

ncbi gb acc num :

NM_001135944.1

uniprot acc num :

Q7KYV8

ncbi mol weight :

5,180 Da

ncbi pathways :

Caspase Cascade In Apoptosis Pathway (137974); Ceramide Signaling Pathway (138023); TNF Receptor Signaling Pathway (138053)

ncbi summary :

Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

uniprot summary :

MADD: a guanine nucleotide exchange factor (GEF) that activates RAB3A/C/D. A GEF for Rab27A/B in melanocytes and during isoproterenol (IPR)-stimulated amylase release from rat parotid acinar cells. Plays a significant role in regulating cell proliferation, survival and death through alternatively spliced isoforms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis). Interacts with the death domain of TNFRSF1A through its own death domain. Highly expressed in fetal brain and kidney, adult testis, ovary, brain and heart. Overexpression of MADD activates the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK). Expression of the MADD death domain stimulates both the ERK and c-JUN N-terminal kinase MAP kinases and induces the phosphorylation of cytosolic phospholipase A2. Isoform 1 is pro-apoptotic, isoform 5 is anti-apoptotic and isoform 3 and isoform 4 have no effect. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines. Isoform 5 is associated with increased cell proliferation and isoform 2 with decreased proliferation. Belongs to the MADD family. Seven isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold; GEFs; GEFs, Rab; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: cytoplasm; integral to membrane; plasma membrane. Molecular Function: protein binding; death receptor binding; Rab guanyl-nucleotide exchange factor activity; protein kinase activator activity. Biological Process: regulation of apoptosis; regulation of Rab protein signal transduction; cell surface receptor linked signal transduction; regulation of cell cycle; activation of MAPK activity

size1 :

0.1 mg

price1 :

370 USD

more info or order :

MyBioSource product webpage