antibody

Anti-FLASH Antibody

MBS194085

0.1 mg

370 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-FLASH Antibody

FLASH (CT)

Anti-FLASH (CT); FLASH (CT)

FLASH; CASP8-associated protein 2; CASP8-associated protein 2; human FLASH; OTTHUMP00000016860; FLASH homolog RIP25; FLICE associated huge; CASP8 associated protein 2; FLICE-associated huge protein; caspase 8 associated protein 2; FLICE-associated huge protein

CASP8AP2; CASP8AP2; CED-4; FLASH; RIP25; FLJ11208; KIAA1315; FLASH; KIAA1315; RIP25

C8AP2_HUMAN

Rabbit

Anti-FLASH (CT) [Rabbit antibody to peptide corresponding to aa 1253-1268 of human FLASH.]. This antibody recognizes human and mouse FLASH (220 kD).

100 ug IgG in 200 ulPBS, pH 7.4, purified by immunoaffinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Immunoblot

Immunoblotting: use at 1:1,000-1:2,000 dilution. Positive control: HeLa cell lysate.

Antigen: Peptide corresponding to aa 1253-1268 of human FLASH. This sequence differs by one amino acid from that of mouse.

Stabilizer: None. Preservatives: 0.02% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Apoptosis Antibodies

A mammalian homologue of CED-4 was recently identified and designated FLASH (FLICE-associated huge protein). FLASH is involved in Fasinduced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH, resulting in activation of caspase-8. FLASH is expressed in multiple tissues and cell types.

6090856

Q9UKL3

222,658 Da

TNF-alpha/NF-kB Signaling Pathway (198884)

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.

CASP8AP2: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF- kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase. Protein type: Nuclear receptor co-regulator; Apoptosis. Chromosomal Location of Human Ortholog: 6q15. Cellular Component: PML body; mitochondrion; cytoplasm; nucleus. Molecular Function: protein binding; DNA binding; SUMO polymer binding; death receptor binding; apoptotic protease activator activity; caspase activator activity; transcription corepressor activity. Biological Process: caspase activation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; cell cycle; signal transduction

0.1 mg

370 USD