antibody

Anti-DFF (CT) Antibody

MBS194063

0.1 mg

370 USD

polyclonal

rabbit

nonconjugated

western blot, immunoprecipitation

Antibody

Anti-DFF (CT) Antibody

DFF (CT) BP

Anti-DFF (CT) BP; DFF45 (CT)

Rabbit

Anti-DFF (CT) BP [Rabbit antibody to peptide corresponding to aa 313-331 at C-terminus of human DNAfragmentation factor DFF.]. This antibody recognizes non-cleaved (45 kD) and cleaved DFF.

100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Immunoblot, Immunoprecipitation

Immunoblotting, Immunoprecipitation : use at 1:1,000-1:2,000 dilution. Positive control: Whole cell lysate from HeLa cells.

Antigen: Peptide corresponding to aa 313- 331 at the C-terminus of human DFF.

Stabilizer: None. Preservatives: 0.02% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Apoptosis Antibodies

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) which is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death.

0.1 mg

370 USD