antibody

Anti-Daxx (CT) Antibody

MBS194057

0.1 mg

370 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-Daxx (CT) Antibody

Daxx (CT)

Anti-Daxx (CT); DAXX (CT)

Daxx; Death-associated protein 6; death domain-associated protein 6; hDaxx; OTTHUMP00000029289; OTTHUMP00000029290; Fas-binding protein; CENP-C binding protein; ETS1-associated protein 1; death-associated protein 6; fas death domain-associated protein; death-domain associated protein

DAXX; DAXX; DAP6; EAP1; BING2; MGC126245; MGC126246

Q4VX54_HUMAN

Rabbit

Anti-Daxx (CT) [Rabbit antibody to peptide at C-terminus of human Fas binding protein DAXX.]. This antibody recognizes full-length human, monkey, and mouse Daxx (120 kD).

100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Immunoblot

Immunoblotting: use at 1:500-1:1,000 dilution. Positive control: Whole cell lysate from HeLa cells.

Antigen: Peptide corresponding to Cterminus of human Daxx.

Stabilizer: None. Preservatives: 0.02% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Apoptosis Antibodies

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain-containing receptors, TNFR1 and Fas. A novel Fas binding protein was recently cloned from human and mouse and designated Daxx. Daxx binds specifically to the Fas death domain, enhances Fas induced apoptosis, and activates the Jun Nterminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues, indicating its important function in Fas signaling pathways.

2253709

AAB63043.1

Q4VX54

19,115 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); FAS Pathway And Stress Induction Of HSP Regulation (198894); HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway (137995); IL-6 Signaling Pathway (198864); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); TGF-beta Receptor Signaling Pathway (198774); TGF-beta Receptor Signaling Pathway (138024)

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described. Protein type: Transcription, coactivator/corepressor; Nucleolus; Apoptosis; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: heterochromatin; PML body; cytoplasm; nucleolus; cytosol; nucleus; chromosome, pericentric region. Molecular Function: protein homodimerization activity; histone binding; p53 binding; protein N-terminus binding; protein kinase binding; transcription factor binding; protein binding; enzyme binding; androgen receptor binding; ubiquitin protein ligase binding; heat shock protein binding; receptor signaling protein activity; transcription corepressor activity; protein kinase activator activity. Biological Process: chromatin remodeling; nucleosome assembly; transcription, DNA-dependent; viral reproduction; cytokinesis after mitosis; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; regulation of protein ubiquitination; apoptosis; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; activation of JNK activity

0.1 mg

370 USD