antibody

Anti-CXCR4 (EL) Antibody

MBS194054

0.1 mg

370 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-CXCR4 (EL) Antibody

CXCR4 (EL)

Anti-CXCR4 (EL); HIV Co-Receptor CXCR4 (EL)

CXCR4; C-X-C chemokine receptor type 4; C-X-C chemokine receptor type 4; fusin; CXC-R4; CXCR-4; CD184 antigen; SDF-1 receptor; OTTHUMP00000204154; OTTHUMP00000204155; neuropeptide Y receptor Y3; seven transmembrane helix receptor; stromal cell-derived factor 1 receptor; lipopolysaccharide-associated protein 3; seven-transmembrane-segment receptor, spleen; leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; chemokine (C-X-C motif) receptor 4; FB22; Fusin; HM89; LCR1; Leukocyte-derived seven transmembrane domain receptor; LESTR; NPYRL; Stromal cell-derived factor 1 receptor

CXCR4; CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E

CXCR4_HUMAN

Rabbit

Anti-CXCR4 (EL) [Rabbit antibody to peptide corresponding to aa 182-196 in the second extracellular loop of human CXCR4.]. This antibody recognizes human and mouse CXCR4 (40 kD).

100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Immunoblot

Immunoblotting: use at 1:500-1:1,000 dilution. Positive control: Whole cell lysate from HeLa cells.

Antigen: Peptide corresponding to aa 182- 196 in the second extracellular loop (EL) of human CXCR4.

Stabilizer: None. Preservatives: 0.02% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Infectious Disease Antibodies

Human immunodeficiency virus (HIV) and related viruses require coreceptors in addition to CD4 to infect target cells. Some G proteincoupled receptors in the chemokine receptor family, including CCR5, CXCR4, CCR3, CCR2b, and CCR8 in the chemokine receptor family, have been identified as HIV coreceptors. CXCR4 (also called Fusin, LESTR, HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD4-independent infection by HIV-2. The alpha-chemokine SDF-1 is the ligand for EXCR4 and prevents infection by T-cell tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. The aminoterminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites. CXCR4 messenger RNA levels correlate with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 may block HIV-1 and HIV-2 infection of human target cells.

3059120

CAA12166.1

P61073

39,746 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Binding And Entry Of HIV Virion Pathway (105986); CXCR4-mediated Signaling Events Pathway (137910); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460)

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

CXCR4: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Monomer. Can form dimers. Interacts with CD164. Interacts with HIV-1 surface protein gp120 and Tat. Interacts with ARRB2; the interaction is dependent on the C-terminal phosphorylation of CXCR4 and allows activation of MAPK1 and MAPK3. Interacts with ARRC; the interaction is dependent on the C-terminal phosphorylation of CXCR4 and modulates calcium mobilization. Interacts (via the cytoplasmic C-terminal) with ITCH (via the WW domains I and II); the interaction, enhanced by CXCL12, ubiquitinates CXCR4 and leads to its degradation. Interacts with extracellular ubiquitin. Interacts with human cytomegalovirus/HHV- 5 protein UL78. Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 2q21. Cellular Component: cell surface; lysosome; early endosome; cytoplasmic membrane-bound vesicle; leading edge; integral to membrane; intercellular junction; growth cone; late endosome; cytoplasm; plasma membrane; cytoplasmic vesicle; external side of plasma membrane. Molecular Function: G-protein coupled receptor activity; viral receptor activity; protein binding; ubiquitin binding; cytokine binding; ubiquitin protein ligase binding; myosin light chain binding; coreceptor activity; actin binding; C-X-C chemokine receptor activity. Biological Process: entry of virus into host cell; regulation of chemotaxis; viral reproduction; apoptosis; activation of MAPK activity; neuron migration; motor axon guidance; regulation of cell migration; germ cell development; T cell proliferation; elevation of cytosolic calcium ion concentration; germ cell migration; dendritic cell chemotaxis; positive regulation of oligodendrocyte differentiation; inflammatory response; neutrophil activation; response to virus; calcium-mediated signaling; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; ameboidal cell migration; myelin maintenance; response to hypoxia; entry into host cell; brain development. Disease: Whim Syndrome

0.1 mg

370 USD