antibody

Anti-CAD (CT) Antibody

MBS194041

0.1 mg

360 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-CAD (CT) Antibody

CAD (CT)

Anti-CAD (CT); CAD (CT)

CAD; DNA fragmentation factor subunit beta; DNA fragmentation factor subunit beta; OTTHUMP00000003633; OTTHUMP00000003634; OTTHUMP00000003649; OTTHUMP00000003673; caspase-activated DNase; caspase-activated nuclease; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; Caspase-activated nuclease; CPAN; DNA fragmentation factor 40 kDa subunit

DFFB; DFFB; CAD; CPAN; DFF2; DFF40; DFF-40; CAD; DFF2; DFF40

DFFB_HUMAN

Rabbit

Anti-CAD (CT) [Rabbit antibody to peptide corresponding to aa 314-329 of mouse CAD.]. This antibody recognizes full-length mouse CAD (40 kD).

100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma-tography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Immunoblot

Immunoblotting: use at 1:500-1:1,000 dilution. Positive control: Mouse lung or spleen tissue lysate.

Antigen: Peptide corresponding to aa 314-329 of mouse CAD.

Stabilizer: None. Preservatives: 0.02% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Apoptosis Antibodies

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (caspase activated deoxyribo-nuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

3410909

BAA32250.1

O76075

39,110 Da

Activation Of DNA Fragmentation Factor Pathway 105683!!Apoptosis Pathway 198797!!Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Apoptosis Pathway 105648!!Apoptosis Induced DNA Fragmentation Pathway 105682!!Apoptotic Execution Phase Pathway 105677!!Caspase Cascade In Apoptosis Pathway 137974!!FAS Pathway And Stress Induction Of HSP Regulation 198894!!HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway 137995

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq]

Function: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. Enzyme regulation: Inhibited by DFFA (DFF45). Subunit structure: Heterodimer of DFFA and DFFB. Subcellular location: Cytoplasm. Nucleus. Sequence similarities: Contains 1 CIDE-N domain.

0.1 mg

360 USD