Anti-Apaf1 (CT) Antibody | MyBioSource MBS194022 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Apaf1 (CT) Antibody

catalog :

MBS194022

quantity :

0.1 mg

price :

370 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS194022

products type :

Antibody

products full name :

Anti-Apaf1 (CT) Antibody

products short name :

Apaf-1 (CT)

products name syn :

Anti-Apaf-1 (CT); Apaf1 (CT)

other names :

apoptotic protease-activating factor 1 isoform e; Apoptotic protease-activating factor 1; apoptotic protease-activating factor 1; apoptotic peptidase activating factor 1

other gene names :

APAF1; APAF1; CED4; APAF-1; DKFZp781B1145; KIAA0413

uniprot entry name :

APAF_HUMAN

host :

Rabbit

specificity :

Anti-Apaf-1 (CT) [Rabbit antibody to peptide corresponding to aa 1158-1177 of human Apaf1. Recognizes human, mouse, and rat Apaf1.]. This antibody recognizes human, mouse, and rat Apaf1 (130 kD).

form :

100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma-tography.

storage stability :

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

tested application :

Immunoblot

app notes :

Immunoblotting: use at 1:500-1:2,000 dilution. Positive control: Human heart tissue lysate.

other info1 :

Antigen: Peptide corresponding to aa 1158-1177 of human Apaf1. The sequence of the immunogenic peptide differs from that of mouse Apaf1 by one amino acid.

other info2 :

Stabilizer: None. Preservatives: 0.02% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

products categories :

Apoptosis Antibodies

products description :

Apoptosis is related to many diseases and induced by a familyof cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.

ncbi gi num :

32483363

ncbi acc num :

NP_863659.1

ncbi gb acc num :

NM_181869.1

uniprot acc num :

O14727

ncbi mol weight :

141,840 Da

ncbi pathways :

Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); Apoptosis Modulation By HSP70 Pathway (198877)

ncbi summary :

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq]

uniprot summary :

APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis. Chromosomal Location of Human Ortholog: 12q23. Cellular Component: cytosol; nucleus; apoptosome. Molecular Function: identical protein binding; protein binding; nucleotide binding; caspase activator activity; ADP binding; ATP binding. Biological Process: caspase activation; regulation of apoptosis; nervous system development; neuron apoptosis; positive regulation of apoptosis; apoptosis; forebrain development; neural tube closure; caspase activation via cytochrome c

size1 :

0.1 mg

price1 :

370 USD

more info or order :

MyBioSource product webpage