antibody

AMPK alpha-2 Polyclonal Antibody

MBS194012

0.1 mg

440 USD

polyclonal

rabbit

nonconjugated

western blot, immunoprecipitation

Antibody

AMPK alpha-2 Polyclonal Antibody

AMPK alpha 2

AMPK alpha 2; AMPK alpha 2

AMP-activated protein kinase alpha 2 catalytic subunit; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK-alpha-2 chain; OTTHUMP00000009993; AMPK subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; protein kinase, AMP-activated, alpha 2 catalytic subunit

PRKAA2; PRKAA2; AMPK; AMPK2; PRKAA; AMPK; AMPK2

AAPK2_HUMAN

Polyclonal

Rabbit

AMPK alpha 2 [Rabbit polyclonal antibody to AMPK alpha 2]. This antibody reacts with human, rat and bovine AMP activated protein kinase, alpha-1 catalytic subunit. Antibody is affinity purified.

100 ug IgG in 0.1 ml Triscitrate/phosphate, pH 7-8

Store at 2 - 8 degree C. Antibody is stable at 2 - 8 degree C for 1 year.

Western Blot, Immunoprecipitation

Western Blot: 0.2 - 2 ug/ml. Immunoprecipitation: 2 - 8 ug/mg lysate

Antigen: Synthetic peptide representing a portion of the protein encoded within exon 7 (LocusLink ID 5563).

Stabilizer: None. Preservatives: 0.1% Sodium Azide

Enzyme and Enzyme Inhibitor Antibodies

47481319

P54646

62,320 Da

AMPK Inhibits ChREBP Transcriptional Activation Activity Pathway 106102!!AMPK Signaling Pathway 198868!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Energy Metabolism Pathway 198907!!Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway 160972!!Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway 160977!!Hypertrophic Cardiomyopathy (HCM) Pathway 114229!!Hypertrophic Cardiomyopathy (HCM) Pathway 106591!!IRS-mediated Signalling Pathway 106426

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq]

Function: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Ref.1. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Enzyme regulation: Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. Subunit structure: Heterotrimer of a catalytic subunit, a beta and a gamma non-catalytic subunits. Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Contains 1 protein kinase domain.

0.1 mg

440 USD