antibody

Clostridium botulinum Toxin A Heavy Chain Monoclonal Antibody

MBS191324

0.5 mg

445 USD

monoclonal

mouse

nonconjugated

ELISA, immunocytochemistry

Antibody

Clostridium botulinum Toxin A Heavy Chain Monoclonal Antibody

[Clostridium botulinum Toxin A Heavy Chain]

Monoclonal

Mouse

This antibody recognizes C. botulinum Toxin A heavy chain. It reacts weakly with Toxin B and E.

Purified antibody in PBS, pH 7.4.

2.5 mg/ml

This antibody is stable for at least one (1) year at-20 degree C to-70 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

End users should determine optimal dilutions for their applications.

Appearance: Clear Liquid. Preservative: None. Antigen: Synthetic peptide corresponding to aa 869-887 of Clostridium botulinum Toxin A heavy chain. Dilution Instructions: Dilute in PBS or medium that is identical to that used in the assay system.

Clostridium botulinum, an anaerobic, gram-positive, spore-forming rod commonly found on plants, in soil, water, and the intestinal tracts of animals, produces eight antigenically distinguishable exotoxins (A, B, C1, C2, D, E, F and G). Type A is the most potent toxin, followed by types B and F. All botulinum neurotoxins are produced as single polypeptide chains of ~150 kDa comprised of a heavy (H) chain and a light (L) chain of roughly 100 and 50 kDa, respectively, linked by a disulfide bond. The heavy (H) chain of the toxin binds selectively and irreversibly to high affinity receptors at the presynaptic surface of cholinergic neurones, and the toxin-receptor complex is taken up into the cell by endocytosis where the disulfide bond between the two chains is cleaved. The light (L) chain interacts with different proteins in the nerve terminals to prevent fusion of acetylcholine vesicles with the cell membrane.

0.5 mg

445 USD