antibody

Anti-Slo2.2 Sodium-Activated K+ Channel Monoclonal Antibody

MBS190600

0.1 mg

420 USD

monoclonal

mouse

nonconjugated

S3-26

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

Antibody

Anti-Slo2.2 Sodium-Activated K+ Channel Monoclonal Antibody

Slo2.2 Sodium-Activated K+ Channel

potassium channel subfamily T member 1; Potassium channel subfamily T member 1; potassium channel subfamily T member 1; potassium channel subunit (Slack); sequence like a calcium-activated potassium channel subunit; potassium channel, subfamily T, member 1; Sequence like a calcium-activated potassium channel subunit

Kcnt1; Kcnt1; Slack; rSlo2; Slack

KCNT1_RAT

Monoclonal

IgG1

S3-26

Mouse

Human, mouse, rat

1168-1237

Slo2.2 Sodium-Activated K+ Channel. This antibody recognizes human (weak), mouse, and rat Slo2.2.

100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence

Immunoblotting: use at 1-10ug/ml. A band of ~140kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate.

Antigen: Fusion protein corresponding to aa 1168-1237 of rat Slo2.2 (accession no. NP_068625). This sequence is 94% homologous with human and 98% homologous with mouse Slo2.2.

Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Slo2.2 is a sodium-activated potassium channel. Slo2.2 channels may contribute to the resting potentials of cells. They also have sensors that couple channel activity to intracellular concentrations of Na+ and Cl-

11177892

NP_068625.1

NM_021853.1

Q9Z258

139,615 Da

Na(+)-activated potassium channel; may be involved in regulating the firing properties of neurons [RGD]

Function: Outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro). Ref.1 Ref.4 Ref.6. Subunit structure: Interacts with CRBN via its cytoplasmic C-terminus. Ref.5. Subcellular location: Cell membrane; Multi-pass membrane protein Ref.3 Ref.5. Tissue specificity: Detected in brain and brainstem, in vestibular and oculomotor nuclei, the medial nucleus of the trapezoid in the auditory system, in olfactory bulb, red nucleus, and deep cerebellar nuclei. Detected in thalamus, substantia nigra, and amygdala (at protein level). Highly expressed in the brain and kidney. Ref.1 Ref.3 Ref.6. Post-translational modification: Phosphorylated by protein kinase C. Phosphorylation of the C-terminal domain increases channel activity. Ref.6. Sequence similarities: Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily. [View classification]Contains 1 RCK N-terminal domain.

0.1 mg

420 USD