antibody

Anti-HCN2 Cyclic Nucleotide-Gated Channel Monoclonal Antibody

MBS190576

0.1 mg

420 USD

monoclonal

mouse

nonconjugated

S71-37

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

Antibody

Anti-HCN2 Cyclic Nucleotide-Gated Channel Monoclonal Antibody

HCN2 Cyclic nucleotide-gated Channel

hyperpolarization-activated, cyclic nucleotide-gated channel 2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; brain cyclic nucleotide gated channel 2; brain cyclic nucleotide-gated channel 2; hyperpolarization activated cyclic nucleotide-gated potassium channel 2; Brain cyclic nucleotide-gated channel 2

HCN2; HCN2; BCNG2; HAC-1; BCNG-2; BCNG2

HCN2_HUMAN

Monoclonal

lgG1

S71-37

Mouse

Mouse, rat

761-863

HCN2 Cyclic nucleotide-gated Channel. This antibody recognizes mouse and rat HCN2. It does not cross-react with HCN1.

100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence

Immunoblotting: use at 1-10ug/ml. A band of ~95kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate

Antigen: Fusion protein aa 761-863 (cytoplasmic C-terminus) of rat HCN2 (accession number Q9JKA9).

Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, play a crucial role in the regulation of cell excitability. They contribute to spontaneous rhythmic activity in the heart and brain.

4996894

Q9UL51

96,950 Da

HCN Channels Pathway 366223!!Potassium Channels Pathway 366221!!Synaptic Transmission Pathway 106513

Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM]

Function: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. Subunit structure: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2. Subcellular location: Membrane; Multi-pass membrane protein. Tissue specificity: Highly expressed throughout the brain. Detected at low levels in heart. Ref.4. Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Miscellaneous: Inhibited by extracellular cesium ions. Sequence similarities: Belongs to the potassium channel HCN family.Contains 1 cyclic nucleotide-binding domain.

0.1 mg

420 USD