catalog number :
MBS190558
products full name :
Anti-Hsp90 Monoclonal Antibody
products short name :
Hsp90
products gene name :
Hsp90
reactivity :
Human, mouse, rat
specificity :
Hsp90. This antibody recognizes human, mouse, and rat Hsp90alpha and beta. Other species have not been tested.
form :
100ug Protein G-purified antibody in PBS, pH 7.4.
storage stability :
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
tested application :
ELISA; Immunoprecipitation; Immunohistochemistry; Immunocytochemistry; Immunoblotting
app notes :
Immunoblotting: use at 0.5-1ug/ml. A band of ~90 kDa is detected. ELISA Immunohistochemistry/ Immunocytochemistry Imunoprecipitation. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate.
other info1 :
Antigen: Recombinant human Hsp90 prufified from E.coli. Preservative: None.
other info2 :
Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
products description :
Hsp90 is an abundantly and ubiquitously expressed heat shock protein found in all eukaryotic cells. It exists in two principal forms, alpha and beta, which share 85% sequence homology. Both forms are expressed in the cytosol. Hsp90alpha exists primarily as a homodimer while Hsp90beta exists mainly as a monomer. Hsp90 plays a role in folding, assembly, maturation, and stabilization of specific proteins as a key component of a chaperone complex. Most of the Hsp90-regulated proteins that have been identified are involved in cell signaling; kinases, v-Src, Wee1, c-Raf, and p53 are some examples. When bound to ATP, Hsp90 interacts with cochaperones Cdc37, p23, and various immunophilin-like proteins to form complexes that stabilize and protect target proteins from proteasomal degradation.
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)