antibody

Anti-Shank1 Monoclonal Antibody

MBS190526

0.1 mg

395 USD

monoclonal

mouse

nonconjugated

S22-21

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

Antibody

Anti-Shank1 Monoclonal Antibody

Shank1

SH3 and multiple ankyrin repeat domains protein 1; SH3 and multiple ankyrin repeat domains protein 1; SH3 and multiple ankyrin repeat domains protein 1; OTTHUMP00000174437; OTTHUMP00000174438; SSTR-interacting protein; somatostatin receptor-interacting protein; SH3 and multiple ankyrin repeat domains 1; Somatostatin receptor-interacting protein

SHANK1; SHANK1; SSTRIP; SPANK-1; synamon

SHAN1_HUMAN

Monoclonal

IgG1

S22-21

Mouse

Human, mouse, rat

469-691

Shank1. This antibody recognizes human, mouse, and rat Shank 1. It does not react with Shank2 or 3.

100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence

Immunoblotting: use at 1-10ug/ml. Bands of ~190-220kDa (alternative splice variants) are detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Adult rat brain.

Antigen: Fusion protein corresponding to aa 469-691 (SH3/PDZ domains) of rat Shank1 (accession no. Q9WV48). This sequence is 96% homologous with human and 100% homologous with mouse Shank1.

Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of postsynaptic density which underlies synaptic dysfunction and loss in Alzheimer's disease. Shank1 might be relevant to human autism spectrum disorders due to its differential role in specific cognitive processes along with its importance in synapse structure and function.

120587025

NP_057232.2

NM_016148.2

Q9Y566

224,959 Da

Glutamatergic Synapse Pathway (213818); Glutamatergic Synapse Pathway (213811)

SHANK1: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Belongs to the SHANK family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: postsynaptic membrane; neuron projection; membrane; cytoplasm; dendrite; plasma membrane; dendritic spine; excitatory synapse; ionotropic glutamate receptor complex; intracellular; cell junction; N-methyl-D-aspartate selective glutamate receptor complex. Molecular Function: protein C-terminus binding; somatostatin receptor binding; identical protein binding; ionotropic glutamate receptor binding; protein binding; protein complex binding; GKAP/Homer scaffold activity; SH3 domain binding. Biological Process: habituation; negative regulation of actin filament bundle formation; synapse maturation; determination of affect; olfactory behavior; long-term memory; righting reflex; cytoskeletal anchoring; adult behavior; protein complex assembly; social behavior; neuromuscular process controlling balance; associative learning

0.1 mg

395 USD