catalog number :
MBS190493
products full name :
Anti-Nitrotyrosine Monoclonal Antibody
products short name :
Nitrotyrosine
products name syn :
Nitrotyrosine
specificity :
Nitrotyrosine [Mouse monoclonal antibody recognizes 3-nitrotyrosine moieties in human, mouse, rat, and dog samples.]. This antibody recognizes 3-nitrotyrosine moieties in human, mouse, rat, and dog samples.
form :
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
storage stability :
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
tested application :
Immunoblot, Immunohistochemistry, Immunoprecipitation
app notes :
Immunoblotting: use at 0.2-2ug/ml. Immunohistochemistry: use at 1-5ug/ml. Immunoprecipitation: use 1-2ug per 100-500ug total protein. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain tissue extract or nitrated BSA.
other info1 :
Antigen: 3-(4-hydroxy-3-nitrophenyl-acetamido) propionic acid conjugated to BSA.
other info2 :
Preservatives: 0.09% sodium azide. Other Additives: 50% glycerol. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
products categories :
Neuroscience and Signal Transduction Antibodies
products description :
Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.
products references :
Girault, I et al. 2001 Free Radic Biol Med 31: 1375-87.