catalog number :
MBS190491
products full name :
Anti-phospho CaMKII Monoclonal Antibody
products short name :
CamKII, phosphorylated
products name syn :
CamKII, phosphorylated
specificity :
CamKII, phosphorylated [Mouse monoclonal antibody recognizes phosphorylated CaMKII.]. This antibody recognizes the phosphorylated form of mouse and rat CaMKII. It does not react with non-phosphorylated CaMKII.
form :
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography
storage stability :
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
tested application :
Immunoblot, Immunoflourescence
app notes :
Immunoblotting: use at 1ug/ml. A band of ~50 kDa is detected. Immunofluorescence: use at 10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain tissue extract.
other info1 :
Antigen: Synthetic peptide based on sequence from rat/mouse CaMKII alpha (aa 281-294), thiophosphorylated at residue Thr286 conjugated to a protein carrier.
other info2 :
Preservatives: 0.09% sodium azide. Other Additives: 50% glycerol. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
products categories :
Neuroscience and Signal Transduction Antibodies
products description :
CaMKII, a member of the calcium / calmodulin-activated protein kinas family, functions in neural synapatic stimulation and T-cell receptor signaling. CaMKII is expressed in many tissues, but it is specifically found in the neurons of the forebrain, and its mRNA is found within the dendrites and the soma of the neuron. CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site. Binding of calcium / calmodulin to its regulatory domain releases its auto-inhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.