catalog number :
MBS190490
products full name :
Anti-CaMKII Monoclonal Antibody
products short name :
CaMKII
products name syn :
CaMKII Antibody; CaMKII
specificity :
CaMKII Antibody [Mouse monoclonal antibody recognizes phosphorylated and non-phosphorylated CaMKII.]. This antibody recognizes the phosphorylated and non-phosphorylated forms of mouse, rat, and bovine CaMKII.
form :
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
storage stability :
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
tested application :
Immunoblot, Immunohistochemistry
app notes :
Immunoblotting: use at 0.1-1ug/ml. A band of ~50-60 kDa is detected. Immunohistochemistry: use at 10ug/ml. RIA: use at 3-5ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain tissue extract.
other info1 :
Antigen: Partially purified rat CaMKII
other info2 :
Preservatives: 0.09% sodium azide. Other Additives: 50% glycerol. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
products categories :
Neuroscience and Signal Transduction Antibodies
products description :
CaMKII, a member of the calcium / calmodulin-activated protein kinas family, functions in neural synapatic stimulation and T-cell receptor signaling. CaMKII is expressed in many tissues, but it is specifically found in the neurons of the forebrain, and its mRNA is found within the dendrites and the soma of the neuron. CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site. Binding of calcium / calmodulin to its regulatory domain releases its auto-inhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.