antibody

Anti-HSF2 Monoclonal Antibody

MBS190478

0.1 mg

345 USD

monoclonal

rat

nonconjugated

western blot, immunohistochemistry, immunoprecipitation

Antibody

Anti-HSF2 Monoclonal Antibody

HSF2

HSF2

heat shock factor protein 2 isoform b; Heat shock factor protein 2; heat shock factor protein 2; OTTHUMP00000017845; heat shock factor 2; heat shock transcription factor 2; Heat shock transcription factor 2

HSF2; HSF2; HSF 2; HSTF 2; MGC75048; MGC117376; MGC156196; HSTF2

HSF2_HUMAN

Monoclonal

IgG1

Rat

HSF2 [Rat monoclonal antibody recognizes HSF2 of multiple species.]. This antibody recognizes HSF2 in heat-shocked cell lysates of human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, sheep, and monkey.

100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Immunoblot, Immunoflourescence, Immunohistochemistry, Immunoprecipitation

Immunoblotting: use at 1-5ug/ml. A band of ~69 kDa is detected. Immunohistochemistry / Immuno-fluorescence: use at 1-5 ug/ml. Immunoprecipitation: use 1-2ug per 100-500ug total protein. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate, K562 cell nuclear extract, human pancreatic tumor cells.

Antigen: Recombinant full-length mouse HSF2

Preservatives: 0.09% sodium azide. Other Additives: 50% glycerol. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Heat Shock and Stress Proteins

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up-regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.

207113147

NP_001129036.1

NM_001135564.1

Q03933

60,348 Da

The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

HSF2: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6q22.31. Cellular Component: cytoplasm; nucleus. Molecular Function: protein homodimerization activity; transcription coactivator activity; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis

0.1 mg

345 USD