catalog number :
MBS190475
products full name :
Anti-Hsp90 alpha Monoclonal Antibody
products short name :
Hsp90alpha
products name syn :
Hsp90alpha
specificity :
Hsp90alpha [Mouse monoclonal antibody recognizes Hsp90alpha of multiple species.]. This antibody recognizes human, mouse, rat, rabbit, bovine, porcine, and chicken Hsp90 alpha (90 kDa).
form :
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
storage stability :
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
tested application :
Immunoblot, Immunoprecipitation
app notes :
Immunoblotting: use at 1-5ug/ml. A band of ~90 kDa is detected. Immunoprecipitation: 5ug on 20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate.
other info1 :
Antigen: Full-length Hsp90 purified from chicken brain
other info2 :
Preservatives: 0.09% sodium azide. Other Additives: 50% glycerol. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
products categories :
Heat Shock and Stress Proteins
products description :
Hsp90 and the 94 kDa glucose-regulated protein, Grp94, are major molecular chapeones of the cytosol and endoplasmic reticulum. In mammalian cells, there are at least two Hsp90 isoforms, Hsp90 alpha and Hsp90 beta , which are encoded by separate genes. All known members of the Hsp90 family are highly conserved, especially in the N-terminal and C-terminal regions. In the absence of stress, Hsp90 is an essential component of cellular processes such as hormone signaling and cell cycle control. Several regulatory proteins such as steroid receptors, cell cycle kinases and p53 have been identified as substrates of Hsp90.
products references :
Schuh S et al. 1985 J Biol Chem 260: 14292-14296; Sullivan WP et al. 1985 Biochemistry 24: 4214-4222.