antibody

Anti-Hsp60 Monoclonal Antibody

MBS190473

0.1 mg

235 USD

monoclonal

mouse

nonconjugated

western blot, immunoprecipitation, flow cytometry

Antibody

Anti-Hsp60 Monoclonal Antibody

Hsp60

Anti-Hsp60; Hsp60

HSP60

Monoclonal

IgG1

Mouse

Anti-Hsp60 [Mouse monoclonal antibody to human Hsp60]. This antibody recognizes human, mouse, rat, rabbit, bovine, canine, porcine, ovine, guinea pig, hamster, chicken, monkey, Xenopus, and Drosophila Hsp60 (60 kDa). It does not cross-react with bacterial or yeast Hsp60. The epitope recognized is within aa 383-447 of human Hsp60.

100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Flow Cytometry, Immunoblot, Immunoprecipitation

Immunoblotting: use at 0.1-1ug/ml. A band of ~60 kDa is detected. Immunoprecipitation: use at 12.5ug/ml. Flow cytometry: use at 10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate.

Antigen: Recombinant human Hsp60 expressed in E. coli.

Preservatives: 0.09% sodium azide. Other Additives: 50% glycerol. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Heat Shock and Stress Proteins

Hsp60 is an abundant protein synthesized constitutively in various cell types that is induced to higher concentrations after cell shock. It is present in mitochondria of many mammalian species and has highly similar counterparts in bacteria and plants (where it is localized to chloroplasts). In general, Hsp60 proteins are present in high concentrations, are induced in response to environmental stresses (such as heat shock) are homo-oligomeric structures of 7 or 14 subunits that dissociate reversibly in the presence of Mg2+ and ATP, have ATPase activity, and play a role in folding and assembly of oligomeric protein structures. Hsp60 has been linked to Alzheimer's disease, coronary artery disease, multiple sclerosis, diabetes, and other autoimmune diseases.

441208

BAA03533.1

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

0.1 mg

235 USD