This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
antibody
product name :
RPA14 Monoclonal Antibody
catalog :
MBS190409
quantity :
0.1 ml
price :
420 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
Saccharomyces cerevisiae S288c
application :
western blot, immunoprecipitation
product information
catalog number :
MBS190409
products type :
Antibody
products full name :
RPA14 Monoclonal Antibody
products short name :
RPA14
products name syn :
Anti-RPA14; RPA14
other names :
Rpa14p; DNA-directed RNA polymerase I subunit RPA14; Rpa14p; DNA-directed RNA polymerase I 14 kDa polypeptide
other gene names :
RPA14; RPA14
uniprot entry name :
RPA14_YEAST
clonality :
Monoclonal
isotype :
IgG1 kappa
host :
Mouse
sequence length :
137
specificity :
Anti-RPA14 [Murine MAb recognizes Replication Protein A (14 kD), a single-stranded DNA binding protein essential in DNA replication, homologous recombination, and nucleotide excision repair.]. This antibody recognizes human and mouse RPA14. RPA (Replication Protein A) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kD. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair.
form :
100 ug Protein G-purified antibody in PBS, pH 7.4
storage stability :
This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
tested application :
Immunoblot, Immunoprecipitation
app notes :
Immunoblotting, immunoprecipitation: use at 0.2-1 ug/ml. Positive control: HeLa, Raji, and 3T3 cells and recombinant protein.
other info1 :
Antigen: N-terminally 6x-His tagged fusion protein corresponding to fulllength human RPA14 expressed in E. coli.
other info2 :
Stabilizer: None. Preservatives: None. Available on request. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
products categories :
Cancer Research Products
products references :
Iftode, C et al. 1999. Crit Rev Biochem Mol Biol 34: 141-180; Bochkarev, A et al. 1999. EMBO 18: 4498-4504; Dong, J et al. 1999. Biochem J 337: 311-317; Sibenaller, ZA et al. 1998. Biochemistry 37: 12496-12506; Stigger, E et al. 1998. J Biol Chem 273: 9337- 9343.
ncbi gi num :
6320360
ncbi acc num :
NP_010440.1
ncbi gb acc num :
NM_001180463.1
uniprot acc num :
P50106
ncbi mol weight :
14,585 Da
ncbi pathways :
Metabolic Pathways 131993!!Purine Metabolism Pathway 870!!Purine Metabolism Pathway 307!!Pyrimidine Metabolism Pathway 871!!Pyrimidine Metabolism Pathway 309!!RNA Polymerase Pathway 946!!RNA Polymerase Pathway 442
uniprot summary :
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. A14 seems to play a role in the stability of subunits ABC23 and A43. In vitro, the A14-A43 subcomplex binds single-stranded RNA. Ref.12. Subunit structure: Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits. Part of a Pol I subcomplex consisting of the subunits A14 and A43. Interacts with RPA43. Ref.9. Subcellular location: Nucleus nucleolus Ref.10. Post-translational modification: The N-terminus is blocked. Miscellaneous: Present with 3100 molecules/cell in log phase SD medium. Ref.11
size :
0.1 ml
price :
420 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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