antibody

Osteonectin/SPARC Monoclonal Antibody

MBS190359

0.1 mg

460 USD

monoclonal

mouse

nonconjugated

ON1-1

western blot, immunohistochemistry, flow cytometry

Antibody

Osteonectin/SPARC Monoclonal Antibody

Osteonectin (SPARC)

Osteonectin (SPARC)

osteonectin; SPARC; SPARC; BM-40; osteonectin; OTTHUMP00000160617; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine

SPARC; SPARC; ON; ON

SPRC_HUMAN

Monoclonal

IgG1

ON1-1

Mouse

303

Osteonectin (SPARC) [Mouse monoclonal antibody to Osteonectin (SPARC)]. This antibody reacts with human and bovine osteonectin as determined by Western Blotting, and cross-reacts with rabbit and porcine osteonectin as determined by ELISA. This antibody reacts with both bone and platelet derived osteonectin; it does not react with non-activated platelets, but it does react with thrombin-stimulated platelets, as determined by Flow Cytometry. treatment with 5mM EDTA for 30 minutes, as determined by Flow Cytometry.

100 ug IgG in 10 mM PBS, pH 7.4, lyophilized.

Lyophilized antibody is stable at 4 degree C for 2 years. Store the reconstituted solution in aliquots at -20 degree C for one year or at 4 degree C for 6 months. Avoid repeated freeze-thaw cycles.

Western Blot, Flow Cytometry, Immunohistochemistry

Western Blotting: 5-10 ug/ml, reducing and non-reducing conditions. Flow Cytometry: 1 ug/ml. Immunohistochemistry: 5-10 ug/ml, paraffin-embedded and frozen tissue sections.

Antigen: Bovine bone osteonectin

Stabilizer: 1% BSA. Preservatives: 0.1% Sodium Azide. Dilutions Instructions: Reconstitute in distilled water to the final desired concentration.

Cell Adhesion Molecule Antibodies

338325

AAA60993.1

P09486

34,632 Da

Formation Of Platelet Plug Pathway 106029!!Hemostasis Pathway 106028!!Platelet Activation Pathway 106034!!Platelet Degranulation Pathway 106050!!Response To Elevated Platelet Cytosolic Ca2+ Pathway 106048!!Senescence And Autophagy Pathway 198780!!TGF-beta Receptor Signaling Pathway 198774

Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).[supplied by OMIM]

Function: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity. Subcellular location: Secreted extracellular space extracellular matrix basement membrane. Note: In or around the basement membrane. Ref.10 Ref.11 Ref.12 Ref.13. Developmental stage: Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. Sequence similarities: Belongs to the SPARC family.Contains 1 EF-hand domain.Contains 1 follistatin-like domain.Contains 1 Kazal-like domain. Sequence caution: The sequence AAA60993.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the C-terminal part.

0.1 mg

460 USD