MyBioSource

antibody

E2F-1 Monoclonal Antibody

MBS190136

0.1 ml

420 USD

monoclonal

mouse

human

western blot, immunoprecipitation

MBS190136

Antibody

E2F-1 Monoclonal Antibody

E2F-1

Anti-E2F-1; E2F-1

E2F-1; Transcription factor E2F1; transcription factor E2F1; PBR3; RBAP-1; RBBP-3; OTTHUMP00000030661; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1; E2F transcription factor 1; PBR3; PRB-binding protein E2F-1; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3

E2F1; E2F1; RBP3; E2F-1; RBAP1; RBBP3; RBBP3

E2F1_HUMAN

Monoclonal

IgG1 kappa

Mouse

Anti-E2F-1 [Murine MAb to GST fusion protein corresponding to aa 223-322 of E2F-1, a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter.]. This antibody recognizes an epitope within aa 223-322 of human E2F-1.

100 ug Protein G-purified antibody in PBS, pH 7.4

This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.

Immunoblot, Immunoprecipitation

Immunoblotting: use at 1-5 ug/ml. Immunoprecipitation: use at 1-5 ug/ml. Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein.

Antigen: GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.

Stabilizer: None. Preservatives: None. Available on request. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Cancer Research Products

181918

Q01094

46,920 Da

Activation Of BH3-only Proteins Pathway 105658!!Activation Of NOXA And Translocation To Mitochondria Pathway 105660!!Activation Of PUMA And Translocation To Mitochondria Pathway 105661!!Adipogenesis Pathway 198832!!Apoptosis Pathway 105648!!Assembly Of The Pre-replicative Complex Pathway 105821!!Association Of Licensing Factors With The Pre-replicative Complex Pathway 105900!!Bladder Cancer Pathway 83115!!Bladder Cancer Pathway 527!!CDC6 Association With The ORC:origin Complex Pathway 105823

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq]

Function: Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. Ref.13 Ref.17 Ref.23. Subunit structure: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A. Interacts with TRIM28; the interaction inhibits E2F1 acetylation through recruiting HDAC1 and represses its transcriptional activity. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity. Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.23. Subcellular location: Nucleus. Post-translational modification: Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Ref.14 Ref.22 Ref.24 Ref.25Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. Sequence similarities: Belongs to the E2F/DP family. Sequence caution: The sequence AAB24289.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

0.1 ml

420 USD