MyBioSource

antibody

Bax Monoclonal Antibody

MBS190066

0.1 mg

380 USD

monoclonal

mouse

rat

western blot

MBS190066

Antibody

Bax Monoclonal Antibody

Bax

Bax

bax; Apoptosis regulator BAX; apoptosis regulator BAX; Bcl2-associated X protein; N/A

Bax; Bax; N/A

BAX_RAT

Monoclonal

IgG1

Mouse

Bax [This mouse monoclonal antibody recognizes aa 12-24 of human, mouse, and rat Bax (20-22 kD), a member of the Bcl-2 family of apoptosis-associated proteins.]. This antibody recognizes human, mouse, and rat Bax (20-22 kD), a member of the Bcl-2 family of apoptosis-associated proteins. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and cell death in a caspase-independent manner.

100 ug Protein G-purified antibody in borate-buffered saline, pH 8.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Immunoblot

Immunoblotting: use at 1-2 ug/ml. In immunoblots, a band of 20-22 kD is detected.

Antigen: KLH-conjugated peptide corresponding to aa 12-24 at the N-terminusof human Bax.

Stabilizer: None. Preservatives: None. Available on request. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Apoptosis Antibodies

975870

Q63690

21,351 Da

ATM Pathway 198524!!Activation, Translocation And Oligomerization Of BAX Pathway 368636!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83491!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 219785!!Apoptosis Pathway 83452!!Apoptosis Pathway 470!!Apoptosis Pathway 368621!!B Cell Receptor Signaling Pathway 198486!!Colorectal Cancer Pathway 83494

Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD]

Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Subunit structure: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Subcellular location: Isoform Alpha: Mitochondrion outer membrane; Single-pass membrane protein. By similarity. Cytoplasm. By similarity. Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. By similarity.Isoform Beta: Cytoplasm. By similarity. Isoform Gamma: Cytoplasm. Tissue specificity: Expressed in a wide variety of tissues, with highest levels in the testis and ovary. Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. Sequence similarities: Belongs to the Bcl-2 family.

0.1 mg

380 USD