antibody

Bax Monoclonal Antibody

MBS190065

0.1 mg

365 USD

monoclonal

mouse

nonconjugated

western blot, immunohistochemistry

Antibody

Bax Monoclonal Antibody

Bax

Bax

bax; Apoptosis regulator BAX; apoptosis regulator BAX; Bcl2-associated X protein

Bax; Bax

BAX_RAT

Monoclonal

IgG1

Mouse

Bax [This mouse monoclonal antibody recognizes aa 3-16 of human Bax (21 kD), a member of the Bcl-2 family of apoptosis-associated proteins.]. This antibody recognizes human Bax (21 kD), a member of the Bcl-2 family of apoptosis-associated proteins. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and cell death in a caspase-independent manner.

100 ug Protein G-purified antibody in borate-buffered saline, pH 8.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Immunoblot, Immunohistochemistry

Immunohistochemistry: use at 5-10 ug/ml on acetone-fixed frozen sections or formalin-fixed paraffin-embedded sections. Heat-induced epitope retrieval is required for formalin-fixed paraffinembedded sections. Immunoblotting: use at 1-2 ug/ml. In immunoblots, a band of 21 kD is detected.

Antigen: KLH-conjugated peptide corresponding to aa 3-16 at the N-terminus of human Bax.

Stabilizer: None. Preservatives: None. Available on request. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Apoptosis Antibodies

975870

Q63690

21,351 Da

ATM Pathway (198524); Activation, Translocation And Oligomerization Of BAX Pathway (368636); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (219785); Apoptosis Pathway (83452); Apoptosis Pathway (470); Apoptosis Pathway (368621); B Cell Receptor Signaling Pathway (198486); Colorectal Cancer Pathway (83494)

Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD]

BAX: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt s lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Belongs to the Bcl-2 family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Apoptosis; Tumor suppressor; Mitochondrial. Cellular Component: mitochondrial permeability transition pore complex; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; cell; nuclear envelope; cytosol; pore complex; mitochondrial outer membrane; membrane; cytoplasm; mitochondrial membrane; intracellular; nucleus. Molecular Function: BH domain binding; identical protein binding; protein binding; protein homodimerization activity; channel activity; protein heterodimerization activity; chaperone binding; heat shock protein binding; protein complex binding; BH3 domain binding; lipid binding. Biological Process: hypothalamus development; regulation of cell cycle; positive regulation of apoptosis; response to toxin; germ cell programmed cell death; myeloid cell homeostasis; homeostasis of number of cells; B cell apoptosis; germ cell development; post-embryonic development; regulation of mammary gland epithelial cell proliferation; spermatid differentiation; regulation of mitochondrial membrane potential; development of secondary sexual characteristics; protein insertion into mitochondrial membrane during induction of apoptosis; regulation of neuron apoptosis; establishment and/or maintenance of transmembrane electrochemical gradient; negative regulation of neuron apoptosis; kidney development; negative regulation of protein binding; response to corticosterone stimulus; response to drug; inner mitochondrial membrane organization and biogenesis; nervous system development; release of cytochrome c from mitochondria; outer mitochondrial membrane organization and biogenesis; positive regulation of B cell apoptosis; regulation of protein homodimerization activity; cellular respiration; vagina development; protein oligomerization; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; negative regulation of fibroblast proliferation; retina development in camera-type eye; reduction of endoplasmic reticulum calcium ion concentration; glycosphingolipid metabolic process; cerebral cortex development; response to ionizing radiation; mitochondrial fragmentation during apoptosis; regulation of nitrogen utilization; post-embryonic camera-type eye morphogenesis; positive regulation of pigmentation; regulation of protein heterodimerization activity; apoptosis; T cell homeostatic proliferation; positive regulation of apoptosis involved in mammary gland involution; negative regulation of peptidyl-serine phosphorylation; neuron migration; regulation of caspase activity; release of matrix enzymes from mitochondria; response to salt stress; negative regulation of cell proliferation; positive regulation of protein oligomerization; apoptotic mitochondrial changes; B cell homeostatic proliferation; B cell homeostasis; ovarian follicle development; positive regulation of neuron apoptosis; response to wounding; response to gamma radiation; response to axon injury; B cell negative selection; protein homooligomerization; leukocyte homeostasis; caspase activation; transformed cell apoptosis; mitochondrial fusion; male gonad development; Sertoli cell proliferation; response to cocaine; limb morphogenesis; odontogenesis of dentine-containing teeth; cell proliferation; neuron apoptosis; response to copper ion; homeostasis of number of cells within a tissue; spermatogenesis; retinal cell programmed cell death; blood vessel remodeling; brain development; caspase activation via cytochrome c; positive regulation of release of sequestered calcium ion into cytosol; sex differentiation; response to DNA damage stimulus

0.1 mg

365 USD