MyBioSource

antibody

Anti-Thymidylate Synthase Monoclonal Antibody

MBS190052

0.2 mg

395 USD

monoclonal

mouse

human

immunoprecipitation, western blot, ELISA, immunohistochemistry

MBS190052

Antibody

Anti-Thymidylate Synthase Monoclonal Antibody

Thymidylate Synthase

Anti-Thymidylate Synthase; Thymidylate Synthase

thymidylate synthase; Thymidylate synthase; thymidylate synthase; TSase; OTTHUMP00000162195; thymidylate synthetase; N/A

TYMS; TYMS; TS; TMS; HST422; MGC88736; TS

TYSY_HUMAN

Monoclonal

IgG1

Mouse

313

Anti-Thymidylate Synthase [Murine MAb to human thymidylate synthase, a drug resistance-associated enzyme.]. These antibodies specifically recognize human TS (36 kDa). They do not crossreact with rat TS.

200 ug Protein G-purified antibody in PBS, pH 7.4.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

ELISA, Immunoblot, Immunohistochemistry, Immunoprecipitation

Immunohistochemistry: use at 5-10 ug/ml on frozen sections (reactivity on paraffin-embedded sections has not been determined). Immunoblotting: use at 5-10 ug/ml. ELISA: use at 2-5 ug/ml (optimized for TS on solid phase at 5 ug/ml). Immunoprecipitation: use #34002 only at 5-10 ug/ml.

Antigen: Recombinant human thymidylate synthase produced in E. coli.

Stabilizer: None. Preservatives: None. Available on request. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Cancer Research Products

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

4507751

NP_001062.1

NM_001071.2

P04818

35,716 Da

Cell Cycle, Mitotic Pathway 105765!!E2F Mediated Regulation Of DNA Replication Pathway 105778!!E2F Transcription Factor Network Pathway 137934!!Fluoropyrimidine Activity Pathway 198778!!G1/S Transition Pathway 105769!!G1/S-Specific Transcription Pathway 105776!!Metabolic Pathways 132956!!Metabolism Of Nucleotides Pathway 106263!!Mitotic G1-G1/S Phases Pathway 160941!!One Carbon Metabolism Pathway 198756

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq]

Catalytic activity: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. Pathway: Pyrimidine metabolism; dTTP biosynthesis. Subunit structure: Homodimer. Sequence similarities: Belongs to the thymidylate synthase family.

0.2 mg

395 USD