Anti-Thymidylate Synthase Monoclonal Antibody | MyBioSource MBS190051 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Thymidylate Synthase Monoclonal Antibody

catalog :

MBS190051

quantity :

0.2 mg

price :

380 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS190051

products type :

Antibody

products full name :

Anti-Thymidylate Synthase Monoclonal Antibody

products short name :

Thymidylate Synthase

products name syn :

Anti-Thymidylate Synthase; Thymidylate Synthase

other names :

thymidylate synthase; Thymidylate synthase; thymidylate synthase; TSase; OTTHUMP00000162195; thymidylate synthetase

other gene names :

TYMS; TYMS; TS; TMS; HST422; MGC88736; TS

uniprot entry name :

TYSY_HUMAN

clonality :

Monoclonal

isotype :

IgG1

host :

Mouse

sequence length :

313

specificity :

Anti-Thymidylate Synthase [Murine MAb to human thymidylate synthase, a drug resistance-associated enzyme.]. These antibodies specifically recognize human TS (36 kDa). They do not crossreact with rat TS.

form :

200 ug Protein G-purified antibody in PBS, pH 7.4.

storage stability :

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

tested application :

ELISA, Immunoblot, Immunohistochemistry, Immunoprecipitation

app notes :

Immunohistochemistry: use at 5-10 ug/ml on frozen sections (reactivity on paraffin-embedded sections has not been determined). Immunoblotting: use at 5-10 ug/ml. ELISA: use at 2-5 ug/ml (optimized for TS on solid phase at 5 ug/ml). Immunoprecipitation: use #34002 only at 5-10 ug/ml.

other info1 :

Antigen: Recombinant human thymidylate synthase produced in E. coli.

other info2 :

Stabilizer: None. Preservatives: None. Available on request. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

products categories :

Cancer Research Products

products description :

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

ncbi gi num :

4507751

ncbi acc num :

NP_001062.1

ncbi gb acc num :

NM_001071.2

uniprot acc num :

P04818

ncbi mol weight :

35,716 Da

ncbi pathways :

Cell Cycle, Mitotic Pathway (105765); E2F Mediated Regulation Of DNA Replication Pathway (105778); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); G1/S Transition Pathway (105769); G1/S-Specific Transcription Pathway (105776); Metabolic Pathways (132956); Metabolism Of Nucleotides Pathway (106263); Mitotic G1-G1/S Phases Pathway (160941); One Carbon Metabolism Pathway (198756)

ncbi summary :

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq]

uniprot summary :

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer. Protein type: Mitochondrial; Nucleotide Metabolism - pyrimidine; DNA repair, damage; EC 2.1.1.45; Methyltransferase; Cofactor and Vitamin Metabolism - one carbon pool by folate. Chromosomal Location of Human Ortholog: 18p11.32. Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; nucleolus; nucleus; cytosol. Molecular Function: mRNA binding; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding. Biological Process: circadian rhythm; methylation; tetrahydrofolate metabolic process; immortalization of host cell by virus; uracil metabolic process; developmental growth; response to organophosphorus; response to toxin; response to glucocorticoid stimulus; dTTP biosynthetic process; response to vitamin A; pyrimidine base metabolic process; response to folic acid; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; aging; response to drug; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; dTMP biosynthetic process; deoxyribonucleoside monophosphate biosynthetic process; G1/S-specific transcription in mitotic cell cycle; response to ethanol; cartilage development; response to cytokine stimulus; pyrimidine nucleoside biosynthetic process; dUMP metabolic process; mitotic cell cycle; response to progesterone stimulus; G1/S transition of mitotic cell cycle

size1 :

0.2 mg

price1 :

380 USD

more info or order :

MyBioSource product webpage