catalog number :
MBS190042
products full name :
Anti-Hsp90 Monoclonal Antibody
products short name :
Hsp90
products name syn :
Hsp90
other names :
HSP90; HSP90
uniprot entry name :
C7F9K9_WUCBA
specificity :
Hsp90 [Mouse monoclonal antibody to Hsp90]. This antibody recognizes human, mouse, and rabbit Hsp90 alpha and Hsp90 beta (90 kDa).
form :
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
storage stability :
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
tested application :
Immunoblot, Immunohistochemistry, Immunoprecipitation
app notes :
Immunoblotting: use at 1-5ug/ml. A band of ~90 kDa is detected. Immunohistochemistry: use at 1-5ug/ml. Immunoprecipitation: 10ug on 10ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate.
other info1 :
Antigen: Purified human Hsp90 beta .
other info2 :
Preservatives: None. Available on request. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
products categories :
Heat Shock and Stress Proteins
products description :
Hsp90 and the 94 kDa glucose-regulated protein, Grp94, are major molecular chapeones of the cytosol and endoplasmic reticulum. In mammalian cells, there are at least two Hsp90 isoforms, Hsp90 alpha and Hsp90 beta , which are encoded by separate genes. All known members of the Hsp90 family are highly conserved, especially in the N-terminal and C-terminal regions. In the absence of stress, Hsp90 is an essential component of cellular processes such as hormone signaling and cell cycle control. Several regulatory proteins such as steroid receptors, cell cycle kinases and p53 have been identified as substrates of Hsp90.
products references :
Barent RL et al. 1998 Mol Endocrinol 12: 342; Felts SJ et al. 2000 J Biol Chem 275: 3305; Arlander SJ et al. 2003 J Biol Chem 278: 52572.
ncbi acc num :
ACT97167.1
ncbi mol weight :
23,689 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)