catalog number :
MBS190039
products full name :
Anti-Hsp70 Monoclonal Antibody
products short name :
Hsp70
products name syn :
Hsp70; Hsp70
other names :
HSP70; HSP70
uniprot entry name :
Q40058_HORVU
specificity :
Hsp70 [Mouse monoclonal antibody to Hsp70]. This antibody recognizes Hsp70 of human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, and C. elegans and does not cross-react with Hsc70. The epitope is in the region of amino acids 436-503 of human Hsp70.
form :
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
storage stability :
This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.
tested application :
ELISA, Flow Cytometry, Immunoblot, Immunohistochemistry, Immunoprecipitation
app notes :
Immunoblotting: 1ug/ml. A band of 70kDa is detected. Immunohistochemistry: 5-10ug/ml. Immunoprecipitation: 1-10ug/ml. ELISA: 1-10ug/ml. Flow cytometry: 10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate.
other info1 :
Antigen: Human Hsp70 purified from HeLa cells.
other info2 :
Preservatives: 0.1% sodium azide. Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
products categories :
Heat Shock and Stress Proteins
products description :
Hsp70 genes, members of a multigene family, encode heat-inducible 70kDa heat-shock proteins that function as molecular chaperones. They have been identified in most organelles of eukaryotic cells as well as in bacteria. The N-terminus of Hsp70 binds ATP with high affinity and the C-terminus binds proteins and polypeptides. Hsp70s bind nascent polypeptides as well as partially folded intermediates of proteins, thus preventing their aggregation and/or misfolding. Binding of ATP triggers a conformational change that results in the release of bound protein. Hsp70s function in protein synthesis, protein folding and oligomerization, and protein transport.
ncbi acc num :
AAA62325.1
ncbi mol weight :
67,016 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Sequence similarities: Belongs to the heat shock protein 70 family. RuleBase RU003322