Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody | MyBioSource MBS179999 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody

catalog :

MBS179999

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human colon, using TOMM20 Antibody(MBS179999). TOMM20 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TOMM20 Antibody (MBS179999)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

image 2 :

Western blot analysis of TOMM20 expression in HepG2 cell lysate (MBS179999). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM20 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TOMM20

product information

catalog number :

MBS179999

products type :

Antibody

products full name :

Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody

products short name :

[TOMM20/Tom20]

products name syn :

[Mitochondrial import receptor subunit TOM20 homolog; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20; TOMM20; KIAA0016]

other names :

[mitochondrial import receptor subunit TOM20 homolog; Mitochondrial import receptor subunit TOM20 homolog; mitochondrial import receptor subunit TOM20 homolog; translocase of outer mitochondrial membrane 20; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20]

products gene name :

[TOMM20]

other gene names :

[TOMM20; TOMM20; MAS20; MOM19; TOM20; KIAA0016]

clonality :

Monoclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

145

purity :

Affinity-chromatography

form :

Liquid; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200. ICC/IF: 1:50-1:200. FC/FACS: 1:100

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human TOMM20. Subcellular Localization: Mitochondrion outer membrane; Single-pass membrane protein.

products categories :

Signal Transduction; Protein Trafficking; Vesicle Transport; regulation; Signal Transduction; Metabolism; Mitochondrial; Metabolism; Pathways and Processes; Mitochondrial Metabolism; mitochondrial biogenesis; Mitochondrial Markers; Types of Disease; Cance

products description :

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity).

ncbi gi num :

7657257

ncbi acc num :

NP_055580.1

ncbi gb acc num :

NM_014765.2

uniprot acc num :

Q15388

ncbi mol weight :

16298 MW

ncbi pathways :

Metabolism Of Proteins Pathway (106230); Mitochondrial Protein Import Pathway (576261)

uniprot summary :

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage