Anti-MEKK3 Rabbit Monoclonal Antibody | MyBioSource MBS179973 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-MEKK3 Rabbit Monoclonal Antibody

catalog :

MBS179973

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[HHC-13]

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of MEKK3 expression in Hela cell lysate (MBS179973). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K3 monoclonal antibody (MBS179973) overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP3K3

product information

catalog number :

MBS179973

products type :

Antibody

products full name :

Anti-MEKK3 Rabbit Monoclonal Antibody

products short name :

[MEKK3]

products name syn :

[Mitogen-activated protein kinase kinase kinase 3; 2.7.11.25; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3; MAP3K3; MAPKKK3; MEKK3]

other names :

[mitogen-activated protein kinase kinase kinase 3 isoform 2; Mitogen-activated protein kinase kinase kinase 3; mitogen-activated protein kinase kinase kinase 3; mitogen-activated protein kinase kinase kinase 3; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3]

products gene name :

[MEKK3]

products gene name syn :

[MAP3K3]

other gene names :

[MAP3K3; MAP3K3; MEKK3; MAPKKK3; MAPKKK3; MEKK3; MEK kinase 3; MEKK 3]

clonality :

Monoclonal

isotype :

IgG

clone :

[HHC-13]

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

657

purity :

Affinity-chromatography

form :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

storage stability :

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

app notes :

WB: 1:500-1:1000. IHC: 1:50-1:200. ICC/IF: 1:50-1:200. IP: 1:20. FC/FACS: 1:50

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human MEKK3. Conjugate: No

products categories :

Signal Transduction; Protein Phosphorylation; SER/THR Kinases; MAPK Pathway; Immunology; Innate Immunity; TLR Signaling

products description :

Anti-MEKK3 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IF, IHC, ICC, WB in Human, Mouse, Rat

ncbi gi num :

42794765

ncbi acc num :

NP_002392.2

ncbi gb acc num :

NM_002401.3

uniprot acc num :

Q99759

ncbi pathways :

Cytokine Signaling In Immune System Pathway (366171); EBV LMP1 Signaling Pathway (198790); EGFR1 Signaling Pathway (198782); GnRH Signaling Pathway (83091); GnRH Signaling Pathway (502); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); IL1-mediated Signaling Events Pathway (137944); Immune System Pathway (106386); Insulin Signaling Pathway (198845)

ncbi summary :

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

uniprot summary :

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage