Anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody | MyBioSource MBS179903 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody

catalog :

MBS179903

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Ubiquitin expression in Jurkat cell lysate (MBS179903). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UBB

product information

catalog number :

MBS179903

products type :

Antibody

products full name :

Anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody

products short name :

[K48-linkage Specific Ubiquitin]

products name syn :

[Polyubiquitin-B; Ubiquitin; UBB]

other names :

[polyubiquitin-B; Polyubiquitin-B; polyubiquitin-B; ubiquitin B]

products gene name :

[UBB]

other gene names :

[UBB; UBB; HEL-S-50]

clonality :

Monoclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

229

purity :

Affinity-chromatography

form :

Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20°C for one year. After reconstitution, at 4° C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)

app notes :

WB: 1:100-1:500. ICC/IF: 1:50-1:200. FC/FACS: 1:50

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human K48-linkage Specific Ubiquitin. Subcellular Localization: Ubiquitin: Cytoplasm. Nucleus.

other info2 :

Conjugate: No

products categories :

Neuroscience; Neurology Process; Neurodegenerative Disease; Alzheimer's Disease; tangles Epigenetics and Nuclear Signaling; Chromatin Modifying Enzymes; ubiquitylation; Cell Biology; Proteolysis/Ubiquitin; Proteasome/Ubiquitin; neuroscience

products description :

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

ncbi gi num :

528524471

ncbi acc num :

NP_001268645.1

ncbi gb acc num :

NM_001281716.1

uniprot acc num :

P0CG47

ncbi pathways :

ABC Transporter Disorders Pathway (1268933); ABC-family Proteins Mediated Transport Pathway (1269904); APC-Cdc20 Mediated Degradation Of Nek2A Pathway (1269847); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (1269838); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (1269848); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (1269849); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (1269844); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (1269851); APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway (1269845); ARMS-mediated Activation Pathway (1269471)

ncbi summary :

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

uniprot summary :

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage