Anti-CD3 epsilon Rabbit Monoclonal Antibody | MyBioSource MBS179879 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CD3 epsilon Rabbit Monoclonal Antibody

catalog :

MBS179879

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[EED-3]

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of CD3 epsilon expression in Jurkat cell lysate (MBS179879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3E monoclonal antibody overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD3E.

image 2 :

IHC analysis of CD3 epsilon using anti- CD3 epsilon antibody (MBS179879) on human colon cancer. CD3 epsilon was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CD3 epsilon Antibody (MBS179879) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (MBS176451) with DAB as the chromogen.

product information

catalog number :

MBS179879

products type :

Antibody

products full name :

Anti-CD3 epsilon Rabbit Monoclonal Antibody

products short name :

[CD3 epsilon]

products name syn :

[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3E; T3E]

other names :

[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3e molecule; T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e]

products gene name :

[CD3E]

other gene names :

[CD3E; CD3E; T3E; TCRE; IMD18; T3E]

clonality :

Monoclonal

isotype :

IgG

clone :

[EED-3]

host :

Rabbit

reactivity :

Human

sequence length :

207

specificity :

The His tag Ab can recognize C-terminal, internal, and Nterminal His-tag fusion proteins.

purity :

Affinity-chromatography

form :

Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

storage stability :

Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200. IP: 1:30. FC/FACS: 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

products categories :

Immunology; Adaptive Immunity; T Cells; Stem Cells; Hematopoietic Progenitors; Hematopoietic Stem Cells; Human Lineage Negative; Immunology; Regulatory T Cells

products description :

The CD3 complex mediates signal transduction.

ncbi gi num :

4502671

ncbi acc num :

NP_000724.1

ncbi gb acc num :

NM_000733.3

uniprot acc num :

P07766

ncbi pathways :

Adaptive Immune System Pathway (366160); CXCR4-mediated Signaling Events Pathway (137910); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (119552); Downstream TCR Signaling Pathway (106418); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Generation Of Second Messenger Molecules Pathway (106417); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

ncbi summary :

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

uniprot summary :

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell developement. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage