Anti-beta III Tubulin Rabbit Monoclonal Antibody | MyBioSource MBS179753 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-beta III Tubulin Rabbit Monoclonal Antibody

catalog :

MBS179753

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human thyroid cancer, using beta III Tubulin Antibody(MBS179753). TUBB3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB3 Antibody (MBS179753)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

image 2 :

Western blot analysis of beta III Tubulin expression in (1) HeLa cell lysate; (2) PC-12 cell lysate; (3) Mouse brain lysate; (4) Rat brain lysate with beta Tubulin III Antibody (MBS179753). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TUBB3

product information

catalog number :

MBS179753

products type :

Antibody

products full name :

Anti-beta III Tubulin Rabbit Monoclonal Antibody

products short name :

[beta III Tubulin]

products name syn :

[Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3; TUBB4]

other names :

[tubulin beta-3 chain isoform 2; Tubulin beta-3 chain; tubulin beta-3 chain; tubulin beta 3 class III; Tubulin beta-4 chain; Tubulin beta-III]

products gene name :

[TUBB3]

other gene names :

[TUBB3; TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A; TUBB4]

clonality :

Monoclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

378

purity :

Affinity-chromatography

form :

Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

app notes :

WB: 1:5000-1:10000. IHC: 1:50-1:200. ICC/IF: 1:50-1:200. IP: 1:50. FC/FACS: 1:50

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human beta III Tubulin. Subcellular Localization: Cytoplasm, cytoskeleton. Tissue Specificity: Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Conjugate: No

products description :

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

ncbi gi num :

308235963

ncbi acc num :

NP_001184110.1

ncbi gb acc num :

NM_001197181.1

uniprot acc num :

Q13509

ncbi pathways :

Chaperonin-mediated Protein Folding Pathway (106242); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway (106243); Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway (106245); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gap Junction Pathway (83072); Gap Junction Pathway (483)

ncbi summary :

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

uniprot summary :

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage