Anti-EAAT2 Rabbit Monoclonal Antibody | MyBioSource MBS179724 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-EAAT2 Rabbit Monoclonal Antibody

catalog :

MBS179724

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[ABED-19]

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of EAAT2 expression in HeLa cell lysate (MBS179724). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC1A2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (MBS176460) with Tanon 5200 system. A specific band was detected for SLC1A2

product information

catalog number :

MBS179724

products type :

Antibody

products full name :

Anti-EAAT2 Rabbit Monoclonal Antibody

products short name :

[EAAT2]

products name syn :

[Excitatory amino acid transporter 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2; SLC1A2; EAAT2; GLT1]

other names :

[excitatory amino acid transporter 2 isoform 2; Excitatory amino acid transporter 2; excitatory amino acid transporter 2; solute carrier family 1 member 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2]

products gene name :

[EAAT2]

products gene name syn :

[SLC1A2]

other gene names :

[SLC1A2; SLC1A2; HBGT; EAAT2; GLT-1; EIEE41; EAAT2; GLT1]

clonality :

Monoclonal

clone :

[ABED-19]

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

563

purity :

Affinity-chromatography

form :

Liquid; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20°C for one year. After reconstitution at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Immunoprecipitation (IP), Western Blot (WB)

app notes :

WB: 1:500-1:2000. IP: 1:50

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human EAAT2

other info2 :

Conjugate: No

products description :

Anti-EAAT2 Rabbit Monoclonal Antibody tested for IP, WB in Human, Mouse, Rat.

ncbi gi num :

307746865

ncbi acc num :

NP_001182657.1

ncbi gb acc num :

NM_001195728.2

uniprot acc num :

P43004

ncbi pathways :

Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway (106533); Glutamatergic Synapse Pathway (213818); Glutamatergic Synapse Pathway (213811); Neuronal System Pathway (106513); Neurotransmitter Uptake And Metabolism In Glial Cells Pathway (106532); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transmission Across Chemical Synapses Pathway (106516)

ncbi summary :

This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2017]

uniprot summary :

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:14506254, PubMed:15265858, PubMed:26690923). Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion (PubMed:14506254). Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport (PubMed:14506254). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate ().

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage