Anti-LAMP2a Rabbit Monoclonal Antibody | MyBioSource MBS179696 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-LAMP2a Rabbit Monoclonal Antibody

catalog :

MBS179696

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human liver cancer, using LAMP2a Antibody(MBS179696). LAMP2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LAMP2 Antibody (MBS179696)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

image 2 :

Western blot analysis of LAMP2a expression in JAR cell lysate (MBS179696). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LAMP2

product information

catalog number :

MBS179696

products type :

Antibody

products full name :

Anti-LAMP2a Rabbit Monoclonal Antibody

products short name :

[LAMP2a]

products name syn :

[Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; Lysosomal membrane glycoprotein type B; LGP-B; CD107b; Lamp2; Lamp-2]

other names :

[lysosome-associated membrane glycoprotein 2 isoform C; Lysosome-associated membrane glycoprotein 2; lysosome-associated membrane glycoprotein 2; lysosomal associated membrane protein 2; CD107 antigen-like family member B; LGP-96]

products gene name :

[LAMP2a]

products gene name syn :

[LAMP2]

other gene names :

[LAMP2; LAMP2; LAMPB; CD107b; LAMP-2; LGP-96; LGP110; LAMP-2; Lysosome-associated membrane protein 2]

clonality :

Monoclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

411

purity :

Affinity-chromatography

form :

Liquid

storage stability :

Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200. IP: 1:50

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human LAMP2a. Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. This protein shuttles between lysosomes, endosomes, and the plasma membrane. Tissue Specificity: Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.

other info2 :

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

products categories :

Tags & Cell Markers; Subcellular Markers; organelles; lysosome; Signal Transduction; Metabolism; Energy Metabolism; Immunology; Secreted Molecules; Other Secreted Molecules; Cardiovascular; Heart; Autophagy; Autophagosome; Stem Cells; Hematopoietic Progen

products description :

Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.

ncbi gi num :

169790833

ncbi acc num :

NP_001116078.1

ncbi gb acc num :

NM_001122606.1

uniprot acc num :

P13473

ncbi pathways :

Hemostasis Pathway (106028); Lysosome Pathway (99052); Lysosome Pathway (96865); Phagosome Pathway (153910); Phagosome Pathway (153859); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Senescence And Autophagy Pathway (198780); Tuberculosis Pathway (213780)

ncbi summary :

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]

uniprot summary :

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032). Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125). Plays a role in lysosomal protein degradation in response to starvation (). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:27628032). Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits (PubMed:20518820). Is not required for efficient MHCII-mediated presentation of endogenous antigens (PubMed:20518820).

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage