product summary
Loading...
company name :
MyBioSource
product type :
antibody
product name :
Anti-Fos B Rabbit Monoclonal Antibody
catalog :
MBS179693
quantity :
0.03 mg
price :
160 USD
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
[DFO-6]
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
image
image 1 :
Immunohistochemical analysis of paraffin-embedded mouse brain, using Fos B Antibody (MBS179693). FOSB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FOSB Antibody (MBS179693) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
image 2 :
Western blot analysis of FosB expression in C6 cell lysate treated with serum (MBS179693). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOSB monoclonal antibody (MBS179693) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (MBS176460) with Tanon 5200 system. A specific band was detected for FOSB.
product information
catalog number :
MBS179693
products type :
Antibody
products full name :
Anti-Fos B Rabbit Monoclonal Antibody
products short name :
[Fos B]
products name syn :
[Protein fosB; G0/G1 switch regulatory protein 3; FOSB; G0S3]
other names :
[protein fosB isoform 2; Protein fosB; protein fosB; FosB proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 3]
products gene name :
[FOSB]
other gene names :
[FOSB; FOSB; AP-1; G0S3; GOS3; GOSB; G0S3]
clonality :
Monoclonal
clone :
[DFO-6]
host :
Rabbit
reactivity :
Human, Mouse, Rat
purity :
Affinity-chromatography
form :
Liquid; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
concentration :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ration to decide dilution procedure.
storage stability :
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze/thaw cycles.
tested application :
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
app notes :
WB: 1:500-1:2000. IHC: 1:50-1:200. ICC/IF: 1:50-1:200. IP: 1:20
image1 heading :
Immunohistochemistry (IHC)
image2 heading :
Western Blot (WB)
other info1 :
Immunogen: A synthesized peptide derived from human Fos B
ncbi gi num :
166999783
ncbi acc num :
NP_001107643.1
ncbi gb acc num :
NM_001114171.1
uniprot acc num :
P53539
ncbi pathways :
Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Cocaine Addiction Pathway (546258); Cocaine Addiction Pathway (546273); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); TGF-beta Receptor Signaling Pathway (198774)
ncbi summary :
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
FosB interacts with Jun proteins enhancing their DNA binding activity.
size1 :
0.03 mg
price1 :
160 USD
size2 :
0.1 mg
price2 :
340
size3 :
0.1 mg + Biotin Secondary (MBS176645)
price3 :
340
size4 :
0.1 mg + HRP Secondary (MBS176703)
price4 :
340
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!
browse more products
questions and comments