product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody
catalog :
MBS179584
quantity :
0.03 mg
price :
160 USD
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry, FACS
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS179584 image 1
Western blot analysis of CHAT expression in SH-SY5Y cell lysate (MBS179584). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHAT monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHAT
product information
catalog number :
MBS179584
products type :
Antibody
products full name :
Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody
products short name :
[CHAT/Choline Acetyltransferase]
products name syn :
[Choline O-acetyltransferase; CHOACTase; ChAT; Choline acetylase; 2.3.1.6; CHAT]
other names :
[choline O-acetyltransferase isoform 1; Choline O-acetyltransferase; choline O-acetyltransferase; choline O-acetyltransferase]
products gene name :
[CHAT]
other gene names :
[CHAT; CHAT; CMS6; CMS1A; CMS1A2; CHOACTASE; CHOACTase; ChAT; Choline acetylase]
clonality :
Monoclonal
host :
Rabbit
reactivity :
Human, Mouse, Rat
sequence length :
630
purity :
Affinity-chromatography
form :
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
storage stability :
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
tested application :
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
app notes :
WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IP: 1:50. FC/FACS: 1:50
image1 heading :
Western Blot (WB)
other info1 :
Immunogen: A synthesized peptide derived from human CHAT
products categories :
Neuroscience; neurotransmitter; acetylcholine; Neuroscience; Cell Type Marker; neuron marker; soma marker
products description :
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
ncbi gi num :
218931221
ncbi acc num :
NP_001136401.1
ncbi gb acc num :
NM_001142929.1
uniprot acc num :
P28329
ncbi pathways :
Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Acetylcholine Synthesis Pathway (198820); Biogenic Amine Synthesis Pathway (198793); Cholinergic Synapse Pathway (217716); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Neurotransmitter Release Cycle Pathway (106518); Synaptic Transmission Pathway (106513); Transmission Across Chemical Synapses Pathway (106516)
ncbi summary :
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
uniprot summary :
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
size1 :
0.03 mg
price1 :
160 USD
size2 :
0.1 mg
price2 :
340
size3 :
0.1 mg + Biotin Secondary (MBS176645)
price3 :
340
size4 :
0.1 mg + HRP Secondary (MBS176703)
price4 :
340
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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