Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody | MyBioSource MBS179560 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody

catalog :

MBS179560

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[ADG-1]

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human kidney, using ARG1 Antibody(MBS179560). ARG1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARG1 Antibody (MBS179560)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(MBS176451) with DAB as the chromogen.

image 2 :

Western blot analysis of ARG1 expression in Mouse liver lysate (MBS179560). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARG1

product information

catalog number :

MBS179560

products type :

Antibody

products full name :

Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody

products short name :

[ARG1/Arginase 1]

products name syn :

[Arginase-1; 3.5.3.1; Liver-type arginase; Type I arginase; ARG1]

other names :

[arginase-1 isoform 2; Arginase-1; arginase-1; arginase 1; Liver-type arginase; Type I arginase]

products gene name :

[ARG1]

other gene names :

[ARG1; ARG1]

clonality :

Monoclonal

clone :

[ADG-1]

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

236

purity :

Affinity-chromatography

form :

Liquid; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

storage stability :

Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Immunoprecipitation (IP), Western Blot (WB)

app notes :

WB: 1:500-1:2000. IP: 1:50

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human ARG1. Conjugate: No

products categories :

Neuroscience; Neurology Process; neuroendocrinology; gh regulation; Biochemicals; Product Range; just add water; Chemical Type; bioactive peptides; Pharmacology; Receptors & transporters; peptide Receptors; ghrelin; agonists

products description :

Anti-ARG1 Rabbit Monoclonal Antibody tested for IP, WB in Human, Mouse, Rat

ncbi gi num :

10947139

ncbi acc num :

NP_000036.2

ncbi gb acc num :

NM_000045.3

uniprot acc num :

P05089

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); IL4-mediated Signaling Events Pathway (137933); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)

ncbi summary :

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

uniprot summary :

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage